放射性^(125)Ⅰ粒子植入对犬正常支气管、食管、肺动脉、肺静脉和肺泡结构的影响及安全性  

Effect of implanted radioactive ^(125)Ⅰ seeds on normal tissue structures of bronchus,esophagus,pulmonary artery,pulmonary vein and alveolus in dogs

作　　者：戚良晨[1] 韩振国[1] 杨斌[1] 何尔斯泰[1] 

机构地区：[1]吉林大学中日联谊医院胸外科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2008年第5期821-824,923,924,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200505159)

摘　　要：目的:研究放射性^(125)Ⅰ粒子植入对犬正常支气管、食管、肺动脉、肺静脉及肺泡组织结构的影响及其安全性,为临床应用提供动物实验证据。方法:将健康雄性杂种犬9只,体重17～21kg,随机分为实验组(30d组和60d组和对照组,每组3只,将3.7×107Bq(1.0mCi)的放射性125I粒子植入犬正常支气管旁、食管旁、肺动脉、肺静脉旁,30d及60d后分别取材,包蜡块进行HE染色光镜观察损伤程度。结果:比较放射性125I粒子植入30d组和60d组犬正常支气管、食管、肺动脉、肺静脉及肺泡组织的损伤,30d组程度低于对照组和60d组,均没有出现穿孔、出血、坏死等并发症。组织病理学改变评分支气管、食管、肺泡改变,30d组和60d组均高于对照组(P<0.05),30d组和60d组间比较差异无显著性(P>0.05);肺静脉的组织病理学改变评分各组间比较差异均无显著性(P>0.05)。结论:放射性125I粒子植入对各组织有不同程度损伤,但这种损伤为可逆性,犬可以通过自身的修复能力修复损伤,临床应用是安全的。Objective To investigate the effect of implanted radioactive ^125Ⅰ seeds on normal tissue structures of bronchus, esophagus, pulmonary artery, pulmonary vein and alveolus in dogs. Methods Nine healthy male dogs weighing 17--21 kg were randomly divided into three groups： 30 d, 60 d experimental groups and control group. Radioactive ^125Ⅰ seeds （3.7×107 Bg, 1.0 mCi） were implanted into the sides of bronchus, esophagus, pulmonary artery, pulmonary vein respectively, the samples of bronchus, esophagus, pulmonary artery, pulmonary vein were taken 30 and 60 d after transplantation, HE staining was used to observe the pathologic changes of the tissues under light microscope. Results The damages of normal bronchus, esophagus, pulmonary artery, pulmonary vein and alveolus after radioactive ^125Ⅰ seeds implantation in 30 d group were weaker than those in control group and 60 d group, there were no complications such as perforation, hemorrhage, necrosis, etc. Histopathological score indicated that the scores of bronchus, esophagus and alveolar in 30 d group and 60 d group were higher than those in control group （P〈0.05）, there was no significant difference between 30 d group and 60 d group （P〉0.05） there was no significant difference in histopathological score of pulmonary vein among all groups （P〉0.05）. Conclusion The implanted radioactive ^125Ⅰ seeds can damage all kinds of tissues at different degrees, but this kind of damage is reversible, the dog may repair the damage through its own repair ability, its clinical application is safe.

关 键 词：放射性粒子 ^125Ⅰ犬 正常组织 支气管 食管 肺动脉 肺静脉 

分 类 号：R815[医药卫生—放射医学]