GHGKHKNK八肽对小鼠黑色素瘤细胞B16-F10侵袭和转移的抑制作用  

Inbitory effect of peptide compound GHGKHKNK on invasion and metastasis of mouse melanoma cell line B16-F10

作　　者：吕刚[1] 田丹[1] 安丽萍[2] 张成义[2] 黎云[3] 孙靖辉[2] 李坦[1] 杨松[2] 高丽囡[2] 张秀娟[2] 李坦诚[2] 杜培革[2] 

机构地区：[1]北华大学生命科学研究中心,吉林吉林132013 [2]北华大学药学院微生物与生化药学教研室,吉林吉林132013 [3]北华大学基础医学院组织学与胚胎学教研室,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2008年第5期825-828,924,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(20050565)

摘　　要：目的:观察GHGKHKNK八肽对小鼠黑色素瘤B16-F10细胞侵袭和转移的抑制作用,为开发具有我国独立的知识产权、针对性强的抗癌小肽类药物奠定临床前的实验基础。方法:MTT法检测GHGKHKNK八肽对小鼠黑色素瘤B16-F10细胞的细胞毒作用;应用C57BL/6J小鼠,尾静脉注射小鼠黑色素瘤B16-F10细胞建立人工肺转移模型,通过免疫组化分析检测GHGKHKNK八肽对小鼠黑色素瘤B16-F10细胞肺转移瘤形成的影响。结果:1×10-4mol.L-1GHGKHKNK八肽作用48h对小鼠黑色素瘤B16-F10细胞的增殖抑制率为17.4%;高剂量组(500μg.kg-1.d-1)GHGKHKNK八肽明显抑制小鼠黑色素瘤B16-F10细胞的肺转移。结论:GHGKHKNK八肽具有抑制小鼠黑色素瘤B16-F10细胞生长和侵袭转移的作用。Objective To observe the inhibitory effect of peptide compound GHGKHKNK on invasion and metastasis of mouse melanoma ceil line B16-F10, in order to exploit a drug with higher pertinency and independent intellectual property rights of our country. Methods Cytotoxic effect of GHGKHKNK on mouse melanoma B16- F10 malignant melanoma cells was evaluated by MTT assay; C57BL/6J mice the were used to establish experimental lung metastasis models by injecting, B16-F10 malignant melanoma cells into the lateral tail vein the effect of GHGKHKNK on experimental lung metastasis in mice was detected with immunohisto chemical analysis. Results The inhibitory of cell 17.4% after treated with 10^-1 mol·L^-1 GHGKHKNK for 48 h. High dose of GHGKHKNK （500μg·kg^-1·d^-1） clearly suppressed lung metastasis in mice. Conolusion GHGKHKNK has a stronger inhibitory effect on invasion and metastasis of B16 F10 malignant melanoma ceils.

关 键 词：GHGKHKNK 黑色素瘤B16-F10细胞 肿瘤转移 C57BL/6J小鼠 

分 类 号：Q78[生物学—分子生物学]