甘肃番茄细菌性斑点病病原菌鉴定  

Pathogen identification of bacterial leaf speck of tomato based on 16S rDNA,physiology and biochemistry in Gansu Province

作　　者：邓刚[1] 屈星[2] 陈秀蓉[1] 杨成德[1] 薛莉[1] 

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,兰州730070 [2]甘肃农业大学农学院,兰州730070

出　　处：《植物保护》2008年第5期47-51,共5页Plant Protection

基　　金：甘肃省星火项目(5HS054-A91-092-02)

摘　　要：采自发病番茄植株上分离获得的菌株,经致病性测定、培养特性、革兰氏染色反应、菌体形态、16S rDNA基因序列分析、生理生化以及寄主范围测定,结果表明:甘肃省张掖市番茄细菌性叶斑病菌为丁香假单胞杆菌番茄致病变种[Pseudomonas syringae pv.tomato(Okabe) Young,Dye&Wikie],为此病的综合防治提供了依据。The strain of the pathogenic bacteria was isolated from the diseased tomato plants in Zhangye, Gansu, and identified as Pseudomonas syringae pv. tomato （Okabe） Young, Dye ＆ Wilkie by pathogenicity, stain reaction, morphological characterization, culture pattern, analysis of 16S rDNA sequence similarity, physiological and biochemical reactions and tests of host range.

关 键 词：细菌性叶斑病 致病性测定 生理生化 16S rDNA鉴定 

分 类 号：S436.412.19[农业科学—农业昆虫与害虫防治]