油菜抗除草剂突变体的筛选及其鉴定  

Selection and identification of herbicide resistant mutants in rapeseed

作　　者：李莉[1] 付岳峰 肖刚[1] 官春云[1] 

机构地区：[1]湖南农业大学农学院 [2]湖南省岳阳市种子公司,湖南岳阳414000

出　　处：《中国油料作物学报》2008年第3期361-365,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家948项目“油菜特异资源和育种新技术的引进、改良及创新研究”(2006-Q04)

摘　　要：利用60Co-γ射线辐照的湘油15油菜种子,以Basta溶液为筛选压在油菜无性系中筛选出了抗除草剂突变体,随后通过打孔叶片抗性鉴定、除草剂喷洒鉴定等方法确定抗性。最后利用SRAP分子标记方法进行鉴定,从而为突变体的真实性提供了分子水平上的证据。Mutagenic treatment was made to seeds of Xiangyou 15 with 60Co - γ/ray. As the result of stepwise positive selection with Basta, mutants were obtained with higher tolerance to herbicide. The herbicide - resistance ability were identified and determined after leaves punched and applied Basta directly. Genetic differences were detected by SRAP markers between the wild type and mutants.

关 键 词：油菜 除草剂 突变体 SRAP 

分 类 号：S565.403[农业科学—作物学]