大鼠胰腺发育中肝细胞因子受体编码基因的表达及蛋白定位  

Expression and protein location of c-met in the different stages of rat pancreatic development

作　　者：程梅[1] 吴玉龙[2] 

机构地区：[1]滨州医学院内科护理学教研室,滨州市256603 [2]滨州医学院病原学教研室

出　　处：《滨州医学院学报》2008年第5期321-323,共3页Journal of Binzhou Medical University

基　　金：教育部科学技术重点研究项目(地方01042);滨州医学院科技计划项目(BY2007KJ05)

摘　　要：目的研究肝细胞因子受体编码基因c-met在大鼠胰腺发育不同阶段的表达及其编码蛋白的细胞定位。方法采用RT-PCR技术检测c-met基因在大鼠胰腺不同发育时期:孕15.5 d(E15.5)和孕18.5 d(E18.5)、生后14 d(P14)、生后21 d(P21)、新生及成年胰腺的表达;并用免疫组化技术检测该基因编码的蛋白c-MET在胰腺发育不同阶段的细胞定位。结果c-met基因在E15.5、E18.5 d较成年特异性高表达,免疫组化结果显示该基因编码的蛋白c-MET在新生后的胰腺大量定位于胰岛细胞。结论c-met可能在胰腺发育过程中起到调控作用,参与胰腺发育中新生后胰岛结构重塑过程。Objective To investigate the expression and location of c-met proto-oneogene in the different development stages of rat pancreas. Methods Using RT-PCR to detect the expression of c-met proto-oncogene in different development stages of rat pancreas： embryonic day 15. 5, embryonic day 18.5, postnatal day 14, postnatal day 21, newborn and adult pancreas. Using immunohistoche to detect the location of the protein of c-met proto-oncogene in the different stages of rat pancreatic development. Results c-met proto-oncogene expresses highly at rat postnatal days, espacially at P14,P21 of rat pancreatic development stages. Conclusion c-met proto-oncogene may play a role in the development of rat pancreas espaciallyin the islet remodation of postnatal rat pancreatic development.

关 键 词：C-MET 胰腺生长发育 RT-PCR 免疫组化 

分 类 号：R318[医药卫生—生物医学工程]