树胶脂毒素对体外培养的背根神经节神经元的影响  

Morphological observations for the toxic effect on the dorsal root ganglion cell of resiniferatoxin

作　　者：于振海[1] 孙毅[1] 张璐萍[1] 阚氏海[1] 刘蛟[1] 黄飞[1] 

机构地区：[1]滨州医学院人体解剖学教研室,滨州市256603

出　　处：《滨州医学院学报》2008年第5期332-334,共3页Journal of Binzhou Medical University

基　　金：滨州医学院科技计划项目(BY2006KJ29)

摘　　要：目的观察树胶脂毒素对体外培养大鼠脊神经背根神经节神经元的影响。方法对Wistar胎鼠背根神经节神经元行原代培养,采用100 nmol/L浓度的树胶脂毒素对培养的神经元进行处理,运用MTT比色法和细胞形态学分析法,观察树胶脂毒素对神经元存活及形态的影响。结果背根神经节神经元存活率降低,神经元呈簇状聚集明显,细胞轮廓增强,胞体缩小,突起断裂或变短甚至消失。结论100 nmol/L浓度的树胶脂毒素对背根神经节神经元有损伤作用。Objective To observe the influence of resiniferatoxin on the dorsal root ganglion of rat. Methods The dorsal root ganglion cell culture model was found, disposed with the resiniferatoxin in the density of 100 nmol/L,and then use the method of MTT and observed the cell＇s morphologie change. Results The resiniferatoxin reduce the survival of the cell. Cell gathered obviously,cell body became smaller,cell processes faulted or shortened or even disappeared. Conclusion The density of the 100 nmol/L resiniferatoxin can damage the dorsal root ganglion cells.

关 键 词：树胶脂毒素 背根神经节 神经元 

分 类 号：R338[医药卫生—人体生理学]