热休克蛋白70过表达对V79细胞辐射损伤的保护作用  

THE PRETECTIVE EFFECTS OF HEAT SHOCK PROTEIN 70 ON RADIATION INJURY OF V79 CELLS

作　　者：秦永春[1] 张保国[1] 洪承皎[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,江苏省放射医学与防护重点实验室,苏州215123

出　　处：《辐射防护》2008年第5期267-270,共4页Radiation Protection

基　　金：国家自然科学基金项目(项目号:10275083)

摘　　要：采用Western blot方法检测进行热休克处理后恢复不同时间的V79细胞中热休克蛋白70(HSP70)的表达情况,采用克隆形成法观察经过热休克预处理的V79细胞经γ射线照射后细胞存活率的变化。实验结果显示:1)V79细胞经42℃热休克预处理恢复1小时后,热休克蛋白70即有明显增加,4小时开始达到高峰,可持续24小时;2)热休克预处理可以提高γ射线照射后V79细胞的存活分数(当照射剂量小于6 Gy时),说明热休克蛋白70对受辐射损伤的V79细胞具有保护作用。Western blot was used to detect the expression of heat shock protein 70 in V79 cells after heat shock pretreatment; V79 cells were irradiated using γ-ray after heat shock pretreatment, survival rate was observed using Colony formation assay. Our study shows that 1 ） the overexpression of heat shock protein 70 was observed in cells recovering for 1 hour after heat shock pretreatment, with peak expression in cells recovering for 4 hours, and could last for 24 hours; 2） heat shock pretreatment was able to elevate survival rate of V79 ceils after irradiation by 660Co γ ray （when the irradiation dose was less than 6 Gy）. The results indicate that heat shock protein 70 has protective effect on radiation induced cell death of V79 cells （when the irradiation dose was less than 6 Gy）.

关 键 词：热休克蛋白70 WESTERN BLOT 克隆形成法 

分 类 号：R144[医药卫生—公共卫生与预防医学]