荧光原位杂交技术在剂量重建中的应用  

APPLICATION OF RE-ESTABLISHING DOSE-RESPONSE CURVES BY FLUORESCENCE IN SITU HYBRIDIZATION(FISH)

作　　者：段志凯[1] 张淑贤[1] 郭万龙[1] 吴启庆[1] 郭鲜花[1] 时爱丽[1] 王斌生[2] 

机构地区：[1]中国辐射防护研究院,太原030006 [2]山西省荣军医院,太原030006

出　　处：《辐射防护》2008年第5期301-304,316,共5页Radiation Protection

基　　金：山西省青年科技研究基金资助项目(编号:22051049)

摘　　要：采用不同剂量60Coγ射线照射两名正常人外周血淋巴细胞后,应用荧光原位杂交(FISH)技术检测染色体易位率,并拟合出剂量效应关系曲线为:Y=0.01316+0.07569D+0.01583D2。通过盲法对实验结果验证,验证结果表明拟合的曲线可用于剂量重建。FISH技术用来检测染色体稳定性畸变,既快速又准确,适合于染色体易位的检测,有望成为剂量重建的理想技术方法。To establish a dose-response relationship between stable chromosome aberration and radiation dose by fluorescence in situ hybridization （FISH）. Translocation frequencies in peripheral blood lymphocytes induced by 6o Coγ-ray at different doses were analyzed by FISH with WCP whole chromosome specific probes for human chromosomes 1, 2 and 4# , and its dose-response curve was fitted. The dose-response formula fitted with translocation frequencies is Y = 0. 01316 ＋ 0. 07569D ＋ 0. 01583D2. After checked by unknown sampies. The formula is certified that it could be used to reestablish dose. Chromosome translocation can be quickly analyzed by FISH and it is hopeful to use translocation frequencies measured by FISH as a dose-response curve re-established biological dosimeter.

关 键 词：荧光原位杂交 稳定性畸变 剂量效应曲线 

分 类 号：Q503[生物学—生物化学]