一种通用型重组2型腺伴随病毒载体的构建及外源基因表达的研究  

CONSTRUCTION OF A UNIVERSAL RECOMBINANT ADENO-ASSOCIATED VIRUS VECTOR AND EXPRESSION OF FOREIGN GENES IN THE VECTOR

作　　者：张桐[1] 王文亮[1] 侯云德[2] 杨新科[2] 颜子颖[2] 孙立连[2] 王伯云[1] 

机构地区：[1]第四军医大学病理学教研室,西安710032 [2]中国预防医学科学院病毒研究所病毒基因工程国家重点实验室,北京100052

出　　处：《西安医科大学学报》1997年第3期312-316,320,共6页Journal of Xi'an Medical University(Chinese)

摘　　要：以野生型2型人腺伴随病毒（AAV－2）基因组载体pSSV9－int-为基础，构建了一种携带和表达真核基因的基础AAV载体pSSV9／MulV－neosv，表达了neo基因，并采用该载体组建了携带人白细胞介素-2cDNA的重组AAV载体pSSV9／MulV－neosv－IL－2，转染A549细胞后表达了人白细胞介素-2基因。为应用腺伴随病毒作基因治疗研究提供了实验基础。Based on a vector, pSSV9-int,which bearing the genome of wild type adeno-associ-ated virus type-2, a universal recombinant AAV vector pSSV9/MulV-neosv was constructed. It was used for cloning and expressing enkaryotic genes.The neo gene in the vector was expressed. Further-MulV-neosv-IL-2, which bears a therapeutic gene,human interleukin-2, was constructed. After trans-fecting A549 cells with the plasmid, the cells pro-duced IL-2. This work provided a foundation for the further research in human gene therapy using the rAAV vectors-

关 键 词：腺伴随病毒 载体构建 基因表达 基因载体 

分 类 号：Q782[生物学—分子生物学] R373[医药卫生—病原生物学]