正常人肝细胞中HBV-PHSA受体基因表达的研究  

Study on PHSAr Gene Expression of HBV in Normal Human Hepatocytes

作　　者：余涛 蔡美英 张子康 

出　　处：《华西医科大学学报》1997年第3期263-267,共5页Journal of West China University of Medical Sciences

基　　金：华西医科大学科研基金

摘　　要：作者根据乙型肝炎病毒（ＨＢＶ）ａｄｒ型ＰｒｅＳ２基因序列设计合成一对聚合酶链反应（ＰＣＲ）引物，经特异性试验后，用于扩增正常人肝细胞中多聚人白蛋白受体（ＰＨＳＡｒ）基因转录产物。结果：ＲＴ－ＰＣＲ及ＲＮＡ－ＰＣＲ扩增结果均为阳性，而相应骨骼肌总ＲＮＡ扩增结果呈阴性，提示乙型肝炎病毒ＰｒｅＳ２区与ＰＨＳＡｒ基因外显子序列具有一定程度的同源性；同时表明ＰＨＳＡｒ基因在肝组织中处于表达状态，而对ＨＢＶ不敏感的组织如骨骼肌则不表达。该研究结果与其它ＨＢＶ－ＰＨＳＡｒ研究相比，进一步证实了ＰＨＳＡｒ在ＨＢＶ感染肝细胞的作用。Based on the sequence of Hepatitis B Virus (type adr) (HBV) Pre S 2 region, we synthesized a pair of PCR primers. After a test of specificity, the primers were employed to amplify the postulated transcripts of PHSAr gene in the liver samples of 3 normal human subjects. By RT PCR and RNA PCR, all amplification results were positive, while those from corresponding skeleton muscle controls were negative. It suggests that HBV Pre S 2 region should be to some extent homologous to PHSAr gene exon(s). It also indicates that PHSAr gene is in expression at transcriptional level, but not in expression in tissues (e.g. skeleton muscle) un susceptible to HBV infection. Our data further confirm the role of PHSAr in HBV infection of hepatocytes, compared with those from membrane receptor researches.

关 键 词：乙型肝炎病毒 肝细胞 PHSA 基因表达 

分 类 号：R373.21[医药卫生—病原生物学] R392.12[医药卫生—基础医学]