海人酸大鼠癫痫模型的建立  

Establishment of epilepsy models in rat by stereotactic injection of kainic acid

作　　者：孟凡刚[1] 张建国[2] 王忠诚[1] 刘阿力[1] 初君盛[2] 张凯[2] 马羽[1] 张斌[2] 张颖[1] 孙异临[1] 

机构地区：[1]首都医科大学北京市神经外科研究所,北京100050 [2]首都医科大学附属北京天坛医院神经外科

出　　处：《中华神经外科杂志》2008年第9期707-709,共3页Chinese Journal of Neurosurgery

基　　金：中国博士后科学基金资助（20060400094）;北京市科技新星计划资助（200B38）

摘　　要：目的应用海人酸注射建立海人酸大鼠癫痫模型，评价其生物学特性。方法通过立体定位手术，大鼠海马组织微量注射海人酸，术后观察大鼠行为学表现、电生理改变及海马超微形态结构变化。结果海人酸注射后实验大鼠出现典型的颢叶癫痫发作过程，表现为湿狗样抖动、前肢抽搐、跌倒以及全身强直一阵挛性发作等，皮层脑电图出现多种形式的痫性放电，单神经元放电细胞外记录显示癫痫模型大鼠放电杂乱，形态不规整。电镜显示神经细胞固缩，星形细胞突起肿胀，神经突触水肿，可见兴奋性递质小泡，线粒体水肿崩解，嵴排列紊乱，血管内皮细胞水肿，血脑屏障破坏。结论大鼠立体定向海人酸药物注射是一种较理想的癫痫模型。Objective To investigate the biological characteristic of temporal lobe epilepsy models in rat models established by injection of kainic acid （KA）. Methods KA was injected into hippocampus of rat by stereotactic operation. Behavior changes, electrophysiological changes and uhrastructural examination were recorded. Results Typical seizure such as wet-dog shakes, convulsion of forelimb, stumble, and clonic-tonic seizure occurred and was recorded by video recording. Electrocorticography （ECoG） showed multiple epileptic waves and extracellular spike recordings of signal neuron showed extensive spikes with irregular shape. Uhrastructural changes of the hippocampus include denaturalization and pyknosis of neuron, swelling of astrocyte and neurite, mitochondria swell, disintegrate with cristal twist, vascular endothelial cells swell and destruction of blood-brain barrier were displayed with electron microscope. Conclusion Epilepsy model in rats established by stereotactic injection of KA can be regarded as one of the ideal models.

关 键 词：癫痫 海人酸 立体定向 动物模型 

分 类 号：R686[医药卫生—骨科学]