Aspergillus fumigatus conidia upregulates NOD2 protein expression both in vitro and in vivo  

作　　者：Hui-jun ZHANG Jie-ming QU Chang-zhou SHAO Jing ZHANG Li-xian HE Zheng-hong YUAN 

机构地区：[1]Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China [2]Department of Pulmonary Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China [3]Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai 200032, China

出　　处：《Acta Pharmacologica Sinica》2008年第10期1202-1208,共7页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （No 30571542） and Shanghai Leading Academic Discipline Project （Project Number： B 115） Author contribution Jie-ming QU, Hui-jun ZHANG, Chang-zhou SHAO, Jing ZHANG, and Li-xian HE designed research; Hui-jun ZHANG performed research; Zheng-hong YUAN contributed new analytical tools and reagents; Hui-jun ZHANG analyzed data; Hui-jun ZHANG wrote the paper.

摘　　要：Aim： To determine if NOD2 is involved in host recognition of Aspergillus fumigatus （Af） conidia. Methods： An Af conidia pulmonary infection murine model was established by intranasal inoculation of Af conidia suspensions. Protein levels of NOD2 in lung tissue were determined by immunohistochemistry. A549 and phorbol-12-myristate 13-acetate （PMA）-activated THP-1 cell lines were treated with heat-killed Af conidia, then the presence of NOD2 protein in these cell lines was detected by Western blotting. The ability of muramyl dipeptide （MDP） to induce the secretion of TNF-α after incubation with heat- killed Afconidia was measured by enzyme-linked immunosorbent assay. Results： The expression of NOD2 protein in lung tissue increased after Af conidia infection. Heat-killed Afconidia significantly upregulated NOD2 protein expression in A549 cells and PMA-activated THP-1 cells. Additionally, Af conidia in conjuction with MDP, significantly increased the secretion of TNF-α in A549 cells and PMA-activated THP-1 cells. Conclusion： Afconidia upregulates NOD2 protein expression in vitro and in vivo. These findings indicate that NOD2 protein may respond to Afconidia.Aim： To determine if NOD2 is involved in host recognition of Aspergillus fumigatus （Af） conidia. Methods： An Af conidia pulmonary infection murine model was established by intranasal inoculation of Af conidia suspensions. Protein levels of NOD2 in lung tissue were determined by immunohistochemistry. A549 and phorbol-12-myristate 13-acetate （PMA）-activated THP-1 cell lines were treated with heat-killed Af conidia, then the presence of NOD2 protein in these cell lines was detected by Western blotting. The ability of muramyl dipeptide （MDP） to induce the secretion of TNF-α after incubation with heat- killed Afconidia was measured by enzyme-linked immunosorbent assay. Results： The expression of NOD2 protein in lung tissue increased after Af conidia infection. Heat-killed Afconidia significantly upregulated NOD2 protein expression in A549 cells and PMA-activated THP-1 cells. Additionally, Af conidia in conjuction with MDP, significantly increased the secretion of TNF-α in A549 cells and PMA-activated THP-1 cells. Conclusion： Afconidia upregulates NOD2 protein expression in vitro and in vivo. These findings indicate that NOD2 protein may respond to Afconidia.

关 键 词：Aspergillus fumigatus NOD2 protein pattern recognition receptors 

分 类 号：R977[医药卫生—药品]