金钱松内生真菌JJ18灭螺活性与菌株鉴定  

Study on molluscicidal effect and identification of endophyte JJ18 from Pseudolarix kaempferi Gord

作　　者：郭尚彬[1,2] 陈钧[1] 王妍[1] 何苗[1] 何晓燕[1] 

机构地区：[1]江苏大学药学院生药学研究室,镇江212013 [2]扬子江医药产业园管理委员会,泰州225321

出　　处：《中国媒介生物学及控制杂志》2008年第5期442-445,共4页Chinese Journal of Vector Biology and Control

基　　金：江苏省2004年度研究生创新计划项目(xm04-57)

摘　　要：目的研究金钱松内生真菌JJ18发酵液醇提物杀螺活性和对湖北钉螺糖原和总蛋白的生理生化影响，并进行菌株鉴定。方法按照世界卫生组织（WHO）的杀螺剂浸泡试验法观察不同浓度下JJ18发酵液提取物的灭螺效果，采用蒽酮显色法测定处理后钉螺软体的糖原含量变化，凯氏定氮法测定其蛋白质含量。采用核糖体rDNAITS区序列分析进行鉴定，并进行系统进化分析。结果JJ18菌株发酵液醇提物灭螺效果显著，试验浓度400mg／L作用3d后灭螺率达到100％，与1mg／L氯硝柳胺溶液处理2～3d的灭螺效果相当；经统计分析：72h灭螺的LC50和LC90浓度分别为145．0和311．9mg／L。JJ18发酵液醇提物处理后钉螺软体的糖原含量显著下降，减少幅度从10．42％～28．03％；蛋白质含量的变化不明显。鉴定该菌为黑曲霉。结论金钱松内生真菌JJ18代谢产物的杀螺活性有开发应用的可能性，显示了植物内生真菌是筛选新型灭螺药物的重要资源。Objective To study the molluscicidal effect of endophyte JJ18 from Pseudolarix kaempferi Cord and its influence on the glycogen and total protein of snails in Hubei, and identify it. Methods The molluscicidal efficacy of different concentration of endophyte JJ18 was detected according to the immersion test method recommended by WHO. The glycogen and protein of Oncomelania hupensis treated by endophyte JJ18 was determined by Anthrone and Kjeldahl method, respectively. J J18 was identified by rDNA ITS sequence. Results The alcohol extract of JJ18 effectively killed O. hupensis. The mortality of O. hupensis was 100% after treatment with 400 mg/L alcohol extract for 3 d, which was similar to that treated with 1 mg/L niclosamidum for 2 - 3 d. Statistical analysis results revealed that the LC50 and LC90 values of the alcohol extract of JJ18 were 145.0 mg/L and 311.9 mg/L after treatment for 72 h, respectively. The content of snail glycogen remarkably decreased after treatment with JJ18 extract, which ranged from 10.42% to 28.03%. The protein content did not significantly change, and JJ18 strain was Aspergillus niger after identification. Conclusion Endophyte JJ18 from P. kaernpferi Cord is a new resource of molluscicide and has the development and application potential, which suggests that endophyte is a important resource to select new molluscicidal compounds.

关 键 词：植物内生真菌 菌株鉴定 灭螺活性 金钱 rDNAITS区 蛋白质含量 湖北钉螺 灭螺效果 

分 类 号：R284.1[医药卫生—中药学] R446.5[医药卫生—中医学]