青藏铁路沿线鼠疫菌外膜蛋白种类的研究  

A study on the outer membrane proteins varieties of the Yersinia pestis along the Qinghai-Tibet railway

作　　者：丛显斌[1] 张春华[1] 赵斌[1] 吕景生[1] 王忠惠[1] 张市[1] 邵奎东[1] 

机构地区：[1]中国疾病预防控制中心鼠疫布鲁氏菌病预防控制基地,白城137000

出　　处：《中国媒介生物学及控制杂志》2008年第5期451-453,共3页Chinese Journal of Vector Biology and Control

基　　金：国家"十五"科技攻关资助项目(2004BA718B08)

摘　　要：目的研究并分析青藏铁路沿线鼠疫菌外膜蛋白种类及相对分子质量。方法鼠疫菌经破碎后采取超高速离心法提取外膜蛋白,利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)系统分离鼠疫菌外膜蛋白。结果在10%的SDS-PAGE上,37℃条件下的培养物可表达26条外膜蛋白带,28℃培养物可表达36条外膜蛋白。结论试验用鼠疫菌株可分泌标准的外膜蛋白,属YOP1型。Objective To study and analyse the molecular weight and outer membrane proteins varieties of the Yersinia pestis. Methods The outer membrane protein was extracted by ultra-centrifuge after the Y. pestis fragmentation and islated by SINS-PAGE electrophoresis. Results There were 26 stripes of outer membrane proteins at 37 ℃ and 36 stripes at 28 ℃ in 10% SDS-PAGE electrophoresis. Conclusion The Y. pestis strains contained standard outer membrane proteins, which belonged to YOP1.

关 键 词：青藏铁路沿线 鼠疫菌 外膜蛋白 

分 类 号：R378.61[医药卫生—病原生物学]