鼠疫F1抗体胶体金检测河北省部分人群血清F1抗体水平的流行病学调查  

Epidemiological investigation on plague F1 antibody at plague natural foci by colloedal gold immunobinding

作　　者：杜国义[1] 史献明[1] 董国润[1] 刘合智[1] 李玉贵[1] 白万翔[1] 乔峰 张月枝[1] 杨顺林[1] 郑金萍 杨建民 齐继东 高少坤 杨小燕[1] 白晓英[1] 胡乐乐[1] 白雪薇[1] 王晓红 吕岩 

机构地区：[1]河北省鼠疫防治所检验科,张家口075000 [2]河北省康保县疾病预防控制中心 [3]河北省蠡县疾病预防控制中心 [4]张家口市桥西区医院

出　　处：《中国媒介生物学及控制杂志》2008年第5期456-457,共2页Chinese Journal of Vector Biology and Control

基　　金：河北省2007年医学科学研究重点课题(07020)

摘　　要：目的了解鼠疫F1抗体胶体金方法的敏感性,分析河北省人群鼠疫F1抗体的分布及产生该分布的原因。方法采集河北省鼠疫自然疫源地历史疫区和动物间鼠疫发生地区(张家口市)健康人群血清样本196份,保定市皮毛加工工人健康人群血清样本162份,非疫区采集100份血清作对照,用鼠疫F1胶体金法进行检测,同时做间接血凝试验,对比其最小检出量。结果通过检测,鼠疫疫源地196份血清检出阳性血清3份,阳性率1.53%;保定市162份血清样本阳性率为0;100份对照血清全部为阴性。结论鼠疫F1抗体胶体金法的最小检出量小于鼠疫间接血凝试验。在鼠疫自然疫源地存在阳性血清,血清阳性者的年龄组成都在40岁以上,保定市皮毛加工工人健康人群检验结果全部为阴性,而非疫区人群血清F1抗体亦为阴性。经过随访,3份阳性血清中,注射鼠疫疫苗产生抗体的有2份,其他不详,需要进一步探讨。Objective To study the sensitivity of colloidal gold immunobinding and analyze the distribution of crowd bubonic plague F1 immune body in Hebei province. Methods 196 serums and 162 serums of healthy people were sampled from history areas affected by plague and plague loci among animals （Zhangjiakou city） in Hebei natural plague foci and fur processing workers in Baoding. 100 serums were collected from non-plague foci as control. Colloedal gold immunobinding and indirect hemagglutination test （IHA） were used to detect, at the same time, the minimum limit of detection of these two methods was compared. Results There were 3 positive serums in 196 serum samples, and the positive rate was 1.53%. None positive samples was found in 162 serum samples. The control were all negative. Conclusion The minimum detection of colloedal gold immunobinding was less than that of IHA. There were positive serum samples in plague natural foci, and the people age whose serum presented positive exceeded 40 years old. The samples collected from Baoding fur processing workers and non-plague foci were all negative. 2 samples were positive after vaccination by investigation, and others needed be further explored.

关 键 词：鼠疫 胶体金免疫实验 流行病学调查 

分 类 号：R254.8[医药卫生—中医内科学]