银屑病患者外周血白细胞mCD14、TLR4和核因子-κB的表达  

Expression of membrane CD14, Toll-like receptor 4 and nuclear factor-kappa B in peripheral blood leukoeytes from patients with psoriasis vulgaris

作　　者：徐丽敏[1] 韩静倩[1] 赵宏丽[1] 李晓莉[1] 

机构地区：[1]天津市长征医院,300120

出　　处：《中华皮肤科杂志》2008年第10期689-691,共3页Chinese Journal of Dermatology

基　　金：天津市卫生局科技基金（07KY10）

摘　　要：目的探讨银屑病患者外周血白细胞mCD14、TLR4和NF-κB表达意义。方法采用流式细胞仪检测银屑病患者32例和正常人29例外周血白细胞mCD14、TLR4和NF-κB的表达。结果32例银屑病组外周血白细胞表面mCD14表达5．454±2．78，对照组3．937±1．964，差异有统计学意义（P〈0．05）。TLR4表达，银屑病组17．641±18．120，对照组7．527±8．574，差异有统计学意义（P〈0．01）。银屑病组外周血总白细胞活化NF-κB的表达为63．538±8．650，对照组62．236±6．72，差异无统计学意义（P〉0．05）。结论银屑病患者中存在mCD14、TLR4表达异常，提示内毒素信号传导通路的异常激活也是感染诱发银屑病的病因之一。Objective To investigate the expression of membrane CD14 （mCD14）, Toll-like receptor 4 （TLR4） and nuclear factor-kappa B （NF-κB） in peripheral blood leukocytes from patients with psoriasis vulgaris （PV）. Methods Blood samples were obtained from 29 normal controls and 32 patients with PV. Patient cohort consisted of 19 males and 13 females, with an average age of 30.28 ± 13.11 years, disease duration of 8.21 ± 10.21 years, and PASI score of 21.19 ± 5.85. Flow cytometry was used to measure the expression of mCD14, TLR4 and activated NF-κB in peripheral blood leukocytes in these subjects. Results The expressions of mCD14 and TLR4 in peripheral blood leukocytes were significantly higher in patients than in the normal controls （5.454 ± 2.78 vs 3.937 ± 1.964, P〈 0.05; 17.641 ± 18.120 vs 7.527 ± 8.574, P 〈 0.01 ）. Although the expression of activated NF-κB in total blood leukocytes in patients was higher than that in normal controls, no statistical difference was noticed （63.538 ± 8.650 vs 62.236 ± 6.72, P 〉 0.05）. Conclusion There is an abnormal expression of mCD14 and TLR4 in patients with PV, suggesting that the abnormal activation of endotoxin signal transduction pathway may exert a role in the induction of psoriasis.

关 键 词：银屑病 抗原 CD14 TOLL样受体4 NF-κB 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]