pH梯度法制备阿霉素脂质体  

Preparation of Adriamycin Liposomes by a pH Gradient Method

作　　者：邓意辉[1] 于彬[1] 李焕秋[1] 顾学裘[1] 

机构地区：[1]沈阳药科大学药物研究所

出　　处：《沈阳药科大学学报》1997年第4期239-242,共4页Journal of Shenyang Pharmaceutical University

摘　　要：采用ｐＨ梯度法制备了阿霉素脂质体．用ＳｅｐｈａｄｅｘＧ－２５微型柱分离－紫外分光光度法测定阿霉素脂质体包封率．结果表明，当药物／磷脂重量比为０．０２～０．０５时，包封率均大于９０％；体外泄漏研究显示，２５℃时，２ｈ内泄漏百分率约为２．７％，保证了临床应用．Adriamycin liposomes(ADML) were prepared by a pH gradient method and the encapsulation efficiency was determined spectrophotometrically by UV absorbance at 480 nm after the free drug molecules was seperated from ADML on a sephadex G 25 minicolumn.The results obtained indicated that the encapsulation efficiency was over 90% when the drug/phospholipid weight ratios were between 0 02 and 0 05.The extent of leakage from liposome was examined by incubation of ADML with 0 9% NaCl at 25℃.The results showed that approximately 2 7% of the entrapped drug was released from ADML within 2 h.

关 键 词：阿霉素 脂质体 PH梯度法 抗肿瘤抗生素 

分 类 号：R979.14[医药卫生—药品] R944.9[医药卫生—药学]