妊娠期糖尿病患者肌肉组织中PI3K介导GLUT4跨膜易位的研究  

Study of phosphatidylinositol-3 kinase mediated glucose transporter-4 translocation in skeletal muscle of patients with gestational diabetes mellitus

作　　者：朱剑文[1] 何明[1] 周燕[1] 谢菡[1] 王娟[1] 王欢[1] 邹丽[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,湖北武汉430022

出　　处：《中国妇幼保健》2008年第29期4182-4185,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金资助(基金编号30600679)

摘　　要：目的:研究妊娠期糖尿病(gestational diabetes mellitus GDM)患者肌肉组织中磷脂酰肌醇3激酶(PI-3K)的活性变化与葡萄糖转运蛋白4(GLUT4)跨膜易位的相关性,探讨它们在妊娠期糖尿病发病机制中的作用。方法:RT-PCR法检测25例GDM患者(GDM组)、26例糖耐量正常孕妇(对照组)肌肉组织中GLUT4 mRNA的表达,western blot法检测各组肌肉组织细胞外膜上GLUT4易位的变化,采用ELISA法检测两组肌肉组织PI-3K的活性,并分析PI-3K的活性变化与GLUT4易位的相关性,采用葡萄糖氧化酶法检测两组空腹血糖(FPG)水平。结果:①基础状态和胰岛素刺激后GDM组和对照组肌肉组织GLUT4 mRNA的表达分别为(0.71±0.02,0.50±0.04)、(0.76±0.03,0.55±0.01),两组比较差异无统计学意义(P>0.05)。②基础状态和胰岛素刺激后GDM组GLUT4跨膜易位分别为(0.61±0.05,0.40±0.03),明显低于对照组(0.93±0.02,0.71±0.04),差异有显著性(P<0.01)。③基础状态和胰岛素刺激后GDM肌肉组织中PI-3K的活性为(3.1±0.2,2.1±0.3),明显低于正常妊娠组(5.2±0.5,3.0±0.6),二者比较差异有显著性,GLUT4跨膜易位与PI-3K活性呈明显正相关(r=0.76,P<0.01)。结论:GDM患者肌肉组织中GLUT4的易位降低与PI-3K的活性下降具有关联性,PI-3K的活性下降是GLUT4的易位降低导致GDM胰岛素抵抗的原因之一,参与了GDM的发病。Objective： To study the relationships between activity of PI- 3K and glucose transporter 4 （GLUT4） translocation in patients with gestational diabetes mellitus （GDM） and explore molecular mechanisms of insulin resistance in GDM. Methods： The expression of GLUT4 mRNA in skeletal muscle tissue were detected by semi quantitative reverse transcription polymerase chain reaction （ RT - PCR） in women with GDM （ GDM group, n = 25）, normal pregnancy women （ control group, n = 26）. The activity of PI - 3K was detected by ELISA assay. GLUT4 translocation in skeletal muscle was detected by Western blotting. Fasting plasma glucose （FPG） was measured by oxidase assay. Results： （1）There was no significant difference in GLUT4 mRNA expression in skeletal muscle between the 2 groups （P 〉 0. 05）. （2）With and without insulin stimulation the translocation of GLUT4 protein were markedly decreased in skeletal muscIe of GDM group （0. 61 ±0. 05, 0. 40±0. 03）compared with control group （0. 93±0. 02, 0. 71±0. 04; P〈0. 01）. （3）With and without insulin stimulation the activity of P1-3K in skeletal muscle of GDM （3. 1 ±0. 2, 2. 1±0. 3） was significantly downregulated compared with those of normal pregnant group （5. 2 ± 0. 5, 3. 0 ± 0. 6 ; P 〈 0. 01） and the translocation of GLUT4 protein was positively related to activity of P1-3K. Conclusion： There are significant correlations of GLUT4 translocation and the decreased activity of PI -3K in skeletal muscle of GDM, may be one of the molecule mechanisms for insulin resistance of GDM.

关 键 词：糖尿病妊娠 胰岛紊抗药性 葡萄糖转运蛋白4 

分 类 号：R714.256[医药卫生—妇产科学]