离体玉米茎尖直接产生雌、雄花序过程中内源激素的研究  

Dynamic Changes of Endogenous Phytohormones in Ears and Tassels Which Emerged from Cultured Shoot Apices of Maize

作　　者：李冬玲[1] 程贯召[1] 

机构地区：[1]潍坊学院生物系,山东潍坊261061

出　　处：《玉米科学》2008年第5期77-79,共3页Journal of Maize Sciences

摘　　要：采用固相抗原型ELISA技术测定了离体玉米茎尖培养过程中3类内源激素的含量。结果表明,在离体培养过程中,3种细胞分裂素(ZRs、DHZRsi、PAs)呈不均衡变化,细胞分裂素总含量在整个离体培养过程中表现出相对较高的浓度。内源IAA随离体培养时间的延长呈下降趋势,其浓度在离体培养第40天时已明显下降;内源GA1/3在离体培养过程中浓度持续增加;内源ABA在整个培养期内含量很低且变化不明显。实验结果显示,IAA的作用可能主要表现在促进玉米茎尖的营养生长和发育,细胞分裂素和GA1/3在离体玉米茎尖由营养生长向生殖生长状态转变的过程中起到了重要的促进作用。Using ELISA（Enzyme Linked Immunosorbent Assay） methods, three-type phytohormone in cultured shoot apices of maize were determined. It showed that the contents of three kinds of endogenous cytokinins changed unbalanced, which including ZRs, DHZRs and iPAs, cytokinins kept in high contents totally compared to the contents of IAA and GAin.The concentration of IAA decreased with the in-vitro culture time was postponed, specially at the time of the 35-40 days when the ears or tassels emerged from the in vitro cultured shoot apices. The contents of GA1/3 increased steadily in all 40 days of the in-vitro culture progress, but the concentration of ABA kept in a very low level in all the in-vitro culture progress. These results exhibited that both GAIn and three kinds of cytokinins played an important and positive role when the maize shoot apices changed from the vegetative developmental stage to the reproductive one.

关 键 词：玉米 内源激素 茎尖 

分 类 号：S513.01[农业科学—作物学]