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机构地区:[1]辽宁石油化工大学环境与生物工程学院,辽宁抚顺113001 [2]沈阳农业大学生物科学技术学院,沈阳110161
出 处:《玉米科学》2008年第5期80-82,86,共4页Journal of Maize Sciences
基 金:国家重点基础研究发展计划(G1999011707)
摘 要:研究在低钾胁迫下玉米苗期体内N、P、K分配和转化酶、可溶性糖分配的规律,探索植物耐低钾胁迫的相关机理。玉米自交系种子A(不耐低钾)和B(耐低钾),用3个不同钾离子浓度(5、100、1 000μmol/L)进行液体培养,4周时将苗转入培养桶,连续培养50 d,测定其体内N、P、K和转化酶、可溶性糖含量。低钾条件下,耐低钾品种B根系中N、K含量显著提高,P含量显著降低,可溶性糖含量增加,转化酶活性与可溶性糖含量呈正相关,转化酶活性随钾浓度升高而上升。低钾胁迫时,耐低钾自交系B根系通过维持较高的N、K含量,增加转化酶活性,从而增加可溶性糖含量来适应低钾胁迫。The article studied the distribution of N, P, K, invertase and soluble sugar in maize under low potassium stress, so that can discover relative mechanism of low potassium stress tolerance. Two inbred lines of maize seed, A which sensitive to K^+ deficiency and B which tolerant to K^+ deficiency were cultured in nutrient solutions of three different K^+ concentration(5, 100, 1 000 μmol/L). After 4 weeks, the maize seedling had been cultured, the plants were transplanted into culture barrel and then cultured for 50 days. Assay the content of N, P, K and invertase, soluble sugar. Results showed that under low K^+ concentration, the content of N and K of B increased significantly while the content of P decreased significantly, and the content of soluble sugar of B increased. The invertase activity is positive correlative with the content soluble sugar, and it ascended by companying with the increasing of potassium concentration. The results indicated that B improved its low potassium stress tolerance through increasing the content of N, K and soluble sugar and the invertase activity.
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