玉米灰斑病菌生理分化鉴定技术比较分析  

Comparison Between Identification Techniques of Physiological Differentiation on Gray Leaf Spot Pathogen

作　　者：王桂清[1] 

机构地区：[1]聊城大学农学院,山东聊城252059

出　　处：《玉米科学》2008年第5期126-130,共5页Journal of Maize Sciences

基　　金：山东省中青年科学家科研奖励基金(2006BS06014);山东省教育厅科学技术项目(J06N01)

摘　　要：比较了玉米灰斑病菌在致病力上的分化、在同工酶水平上的分化和在DNA水平上的分化。结果表明,玉米灰斑病菌部分菌株的致病力与蛋白质及多种同工酶谱带有一定的相关性,但蛋白质和同工酶的变化还不能完全反映所有菌株致病力的分化;玉米灰斑病菌的RAPD标记和致病力之间相关性较小,在鉴定具有明显致病力分化的菌株时结果较理想。Compared with the differentiation of gray leaf spot（GLS）pathogen in virulence, isozyme level and DNA level ,we drew the conclusion that there existed certain correlation between the ＂virulence of some isolated from GLS pathogen and their isozyme spectrums. However, the variation of isozymes could not represent the dif- ferentiation of all isolates from GLS pathogen in virulence completely. The correlation between RAPD labels and virulence of GLS pathogen were smaller, so it could get more perfect results when identifying the isolated with obvious differentiation in virulence.

关 键 词：玉米灰斑病菌 致病力 同工酶 随机扩增多态性DNA 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]