玉米丝黑穗病菌的PCR检测  

PCR Detection of Sporisorium Reilianum

作　　者：任林柱[1] 李莉[1] 白容霖[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062

出　　处：《玉米科学》2008年第5期138-139,共2页Journal of Maize Sciences

基　　金：吉林大学农学部青年创新基金项目

摘　　要：根据玉米丝黑穗病菌pra3基因序列设计了1对扩增玉米丝黑穗病菌的特异引物。结果显示,用该特异引物扩增玉米丝黑穗病菌DNA可以得到一条大小为499bp的目的条带,扩增玉米丝黑穗病菌DNA的灵敏度可以达到1pg,建立的PCR反应可用于玉米丝黑穗病菌的检测。A pair of species-specific primer for sporisorium reilianum was designed according to the pra3 gene of sporisorium reilianum, and the results of polymerase chain reaction showed that a 499bp DNA was amplified by using the primers, and the PCR-hased assay could detect more than lpg fungal DNA using the primer. The PCR as a highlysensitive and reliable tooi, Could be used in detection of sporisorium reilianum.

关 键 词：玉米丝黑穗病菌 聚合酶链式反应(PCR) 检测 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]