妊娠压力与铅联合暴露对大鼠子代空间学习记忆的影响  

Effects of prenatal exposure to stress and lead on spatial learning and memory development in rats

作　　者：李筑英[1] 颜崇淮[1] 徐健[1] 黄华[1] 邹向宇[1] 

机构地区：[1]上海交通大学医学院附属新华医院上海市儿科医学研究所上海市环境与儿童健康重点实验室,200092

出　　处：《中华预防医学杂志》2008年第10期717-721,共5页Chinese Journal of Preventive Medicine

基　　金：上海市科委重大基础研究课题（05DJ14007）

摘　　要：目的探讨妊娠压力与铅联合暴露对大鼠子代早期空间学习记忆能力的影响。方法运用数字表法随机将32只Sprague-Dawley孕鼠分为空白对照组（NS／C），铅暴露组（NS／L），压力暴露组（S／C），联合暴露组（S／L），每组8只。NS／L、S／L自由饮用0．2％醋酸铅溶液，S／C、S／L给予束缚压力。子代于30d龄进行Morris水迷宫测试，并检测海马组织铅含量及水迷宫实验前、后血清肾上腺酮。结果S／L雄、雌性仔鼠在原平台所在象限停留时间分别为（16．08±3．41）s、（15．72±3．33）s，显著短于NS／L（25．42±4．76）s、（24．55±4．43）s和S／C（20．96±3．45）s、（20．65±2．98）s，妊娠压力与铅对仔鼠在原平台所在象限停留时间的影响存在交互作用（F=5．478，P〈0．05）；妊娠压力与铅对仔鼠应激后血清肾上腺酮水平的影响存在交互作用。NS／L、S／L仔鼠海马铅含量分别为（0．4378±0．1041）μg／g、（0．4679±0．1243）μg／g，差异无统计学意义（F=0．298，P〉0．05）。结论（1）妊娠压力与铅对大鼠子代学习记忆的损害可能具有叠加作用。（2）妊娠压力与铅对仔鼠下丘脑．垂体一肾上腺轴的影响可能具有叠加作用。该作用可能是二者对子代学习记忆叠加损害的原因之一。（3）联合暴露未显著增加铅在子代海马中的蓄积。Objective To explore the effects of prenatal exposure to stress and lead on spatial learning and memory development in rats. Methods All 32 Sprague-Dawley （SD） pregnant rats were divided randomly into 4 groups, 8 per group in line with the Random Number Table. The four groups were： no maternal stress, no Pb exposure （NS/C） ; non-maternal stress, Pb exposure （NS/L）, maternal stress, no Pb exposure （S/C） , and maternal stress plus Pb exposure （S/L）. The spatial learning and memory ability, the serum corticosterone level both pre and post-testing of 30-day old offsprings, and lead concentration in hippocampus were tested by means of Morris Water Maze, radioimmunoassay and Inductively Coupled Plaswa Mass Spectrometry（ICP-MS）. Results The residence time of male and female in NS/L was （ 16. 08± 3.41 ） s, （ 15.72 ± 3.33 ） s, which were significantly shorter than NS/L （ 25.42 ± 4. 76） s, （24. 55 ± 4.43 ） s and S/C （ 20. 96 ± 3.45 ） s, （ 20. 65 ± 2. 98 ） s, and significant difference was observed in the joint exposure effect（ F = 5.478, P 〈 0.05 ）. The effect of the joint exposure was significant on post-testing serum corticosterone. The hippocampus lead concentrations of NS/L and S/L were （0. 4378 ±0. 1041 ） μg/g and （ 0. 4679 ±0. 1243 ） μg/g without significant differences （ F = 0. 298, P 〉 0.05 ）. Conclusion Prenatal joint exposure to restraint stress and lead might increase the effects of single exposure on the spatial learning and memory ability and serum corticosterone level of offsprings, and the joint influence on corticosterone level might be one of the reasons of further impairment of learning and memory.

关 键 词：妊娠压力 铅 环境暴露 迷宫学习 记忆 

分 类 号：R686[医药卫生—骨科学]