大气细颗粒物水溶成分对大鼠胸主动脉舒缩功能的影响  

The comparison study on effects of water-soluble components of fine particulate matter on vasomotor function in aortas from rats after exposure in different time

作　　者：秦纲[1] 张明升[2] 王德锁 梁月琴[2] 药红梅[1] 齐守罡[1] 吕吉元[1] 

机构地区：[1]山西医科大学第一医院心内科,太原030001 [2]山西医科大学药理教研室 [3]美国北卡罗来纳州国家环境健康科学研究所

出　　处：《中华预防医学杂志》2008年第10期762-765,共4页Chinese Journal of Preventive Medicine

基　　金：山西省科技攻关项目（20080311060-5）

摘　　要：目的探讨大气细颗粒物（PM2.5）水溶成分静脉染毒后1、6h，对大鼠胸主动脉血管环收缩、舒张功能的影响。方法雄性Wistar大鼠30只，分为空白对照组、1h对照组、6h对照组、染毒1h组、染毒6h组，每组6只。染毒组以PM2.5水溶成分（1ml／kg）尾静脉注射1、6h后，分别以25％乌拉坦腹腔注射（0．4ml／100g）麻醉后，迅速开胸取大鼠胸主动脉，制备血管环，置于张力换能器进行实验。1h、6h对照组以0．9％氯化钠注射液（1ml／kg）尾静脉注射，处理同染毒组。结果空白对照组、1h对照组、6h对照组三组间血管环舒缩反应差别无统计学意义。染毒1h后，胸主动脉血管环对60mmol／L KCl的收缩反应减弱，6h后有升高趋势，但仍低于对照组。10^-6mol／L去甲肾上腺素（NE）预收缩的血管环：乙酰胆碱（ACh）浓度在10^-5mol／L、10^-1mol／L水平，染毒后1h舒张率降低。ACh浓度在10～～10一’mol／L水平，染毒6h组舒张率较染毒1h组升高；硝普钠（SNP）浓度在10^-5～10^-9mol／L水平，染毒后1h舒张率降低，在10^-6mol／L、10^-9mol／L水平，染毒后6h组舒张率较染毒1h组升高，在10^-5～10^-7mol／L水平，染毒6h组舒张率仍低于对照组。结论PM2.5水溶成分静脉染毒1h后引起血管收缩抑制、舒张下降，6h后该作用减弱。Objective To compare the difference of vasomotor functions in aortas segments from Wistar rats between 1-hour and 6-hours after exposure of water-soluble components of fine particulate matter （PM2.5）- Methods All 30 Wistar rats were assigned to five groups （n = 6 for each group） at random： the blank control group, control group for 1-hour and 6-hours, exposure group for 1-hour and 6-hours. The rats were sacrificed 1-hour or 6-hours later and aorta ring segments were mounted on wire myographs. Results （ 1 ） There was no significant difference in vasomotor functions among three control groups （ P 〉 0. 05 ）. （2） 1-hour or 6-hours after exposure there was a decrease of contraction elicited by 60 mmol/L KCl in contrast to the control group（P 〈 0.05 ）, whereas no significant change between the exposure group for 1-hour and 6- hours （P 〉0. 05）. （3）On the level of 10^-5 or 10^-7 mol/L, 1-hour after exposure there was a decrease in endotbelium-dependent acetylcholine（ACh） elicited relaxation precontracted by 10^-6mol/L NE compared with the control group （P〈0.01 or P〈0. 05）, on the level from 10^-5 to 10^-7 mol/L there was a decrease compared with the exposure group for 6-hours （ P 〈 0. 05 ）, whereas no difference between the exposure group for 6-hours and the control group （ P 〉 0. 05 ）. On the level from 10^-5 to 10^-9 mol/L, 1-hour after exposure there was a decrease in endothelium-independent sodium nitroprusside （SNP）elicited relaxation precontracted by 10^-6mol/L NE as compared with the control group（P 〈 0. 01 or P 〈 0. 05 ）and a decrease on the level of 10^-6 or 10^-9 mol/L compared with the exposure group for 6-hours （ P 〈 0. 05 ）, 6-hours after exposure a decrease was caused as compared with the control group on the level from 10^-5 to 10^-7 mol/L （P 〈 0. 01 or P 〈 0. 05 ）. Conclusions Inhibition of contraction and impairment of relaxation in aortas should be caused 1-hour after exposure to water-soluble components of PM2.5 i

关 键 词：空气污染物 颗粒物 胸主动脉 大鼠 

分 类 号：R686[医药卫生—骨科学]