黄芪失笑散对实验大鼠主动脉和OX-LDL诱导下人脐静脉内皮细胞ICAM-1 VCAM-1 mRNA基因表达的影响  

Effect of Huangqi Shixiao Powder on ICAM-1 VCAM-1 Expression of HUVEC_S Exposed to Oxidized Low-density Lipoprotein and AS Rat

作　　者：祝光礼[1] 魏丽萍[1] 方伟[1] 钱宝庆[1] 赫小龙[1] 

机构地区：[1]浙江中医药大学附属广兴中医院,浙江杭州310007

出　　处：《中华中医药学刊》2008年第10期2095-2098,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药管理局资助课题(2006C107)

摘　　要：目的：探讨黄芪失笑散对动脉硬化大鼠主动脉和氧化低密度脂蛋（OX—LDL）刺激下人脐静脉内皮细胞（HUVECs）细胞间黏附分子-1（ICAM—1）、血管黏附分子-1（VCAM—1）mRNA基因表达影响。方法：采用高脂饲料喂饲和腹腔注射维生素D，建立大鼠AS模型，取48只雄性SD大鼠随机分为6组：正常对照组、模型组、氟伐他汀组、黄芪失笑散小剂量组、黄芪失笑散中剂量组、黄芪失笑散大剂量组。治疗12周后，用RT—PCR法检测大鼠主动脉ICAM—1、VCAM—1的表达；进行HUVECs原代培养，取第4～6代进行实验，随机分为6组：对照组、模型组、氟伐他汀组、黄芪失笑散小剂量组、黄芪失笑散中剂量组、黄芪失笑散大剂量组。OX—LDL刺激HUVECs6h后，大鼠药物血清与HUVECs共培养48h，用RT—PCR法检测大鼠主动脉ICAM—1、VCAM—1的表达。结果：黄芪失笑散大剂量可显著降低动脉粥样硬化大鼠主动脉、OX—LDL诱导的HUVECs的ICAM—1、VCAM—1 mRNA表达（P〈0．01），且黄芪失笑散对OX—LDL诱导的HUVECs的ICAM-1、VCAM-1 mRNA表达的抑制作用呈剂量依赖性。结论：黄芪失笑散的抗动脉粥样硬化的作用源于抑制ICAM-1、VCAM-1 mRNA的表达。Abstract： Obejective： To explore the effects of Huangqi Shixiao Powder on ICAM - 1, VCAM - 1 expression HU- VECs exposed to oxidized low- density lipoprotein （OX -LDL） and atherosclerotic （AS） rats. Methods ：The atheroscle- rosis （AS） animal model was established in SD rats by high grease food feeding and VitD3 injection except the control group. The 48 male SD rats were randomly divided into control group, model group, Fluvastatin group, low - dose Huangqi Shixiao Powder group, mid - dose Huangqi Shixiao Powder group, high - dose Huangqi Shixiao Powder.Twelve weeks later,ICAM - 1 ,VCAM -1 were detected by reverse transcriptase polymerase chain reaction（ RT- PCR） in rat aorta. The HUVECs were primary cultured. HUVECs from third to sixth generations were randomly divided into control group（ model group, Fluvastatin group ,low - dose Huangqi Shixiao Powder group, mid - dose Ituangqi Shixiao Powder group,high - dose Ituangqi Shixitw Powder） and stimulated with OX - LDL for six hours, then HUVECs were cultured with Drug-contained serum for 48 hours . The ICAM - 1 ,VCAM - 1 were detected by reverse tranacriptaae polymerase chain reaction（ RT - PCR） in HUVECs. Results ： The high - dose Huaugqi Shixiao Powder can significantly inhibit ICAM - 1 ,VCAM - 1 expression in HUVECs exposed to oxidized low - density lipoprotein（ OX - LDL） and aorta of atherosclerotic rats. Huangqi Shixiao Powder has a concentration - dependent inhibition effect in HUVECs injured by OX - LDL. Conclusion： Huaugqi Shixiao Powder has anti - atherogenic effect due to inhibit the expression of ICAM - 1 and VCAM - 1.

关 键 词：动脉粥样硬化 ICAM—1 VCAM-1 人脐静脉内皮细胞 氧化低密度脂蛋白 

分 类 号：R285.5[医药卫生—中药学]