海昆肾喜对糖尿病肾病大鼠肾脏保护作用及其对肾组织VEGF蛋白表达的影响  

Effect of Haikun Shenxi on Vascular Endothelial Growth Factor Protein Expression in Kidney and Protective Effect of Kidney in Diabetic Rats

作　　者：李杨[1] 赵宗江[1] 杨美娟[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中华中医药学刊》2008年第10期2164-2166,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：教育部"长江学者和创新团队发展计划"资助项目(IRT0413);国家自然科学基金重点资助项目(30130220);高等学校学科创新引智计划资助项目(B07007)

摘　　要：目的:探讨海昆肾喜对糖尿病肾病大鼠肾组织血管内皮生长因子(VEGF)蛋白表达的影响。方法:60只大鼠随机分为两组,正常组10只,造模组50只。造模组采用空腹一次性腹腔注射链脲佐菌素造成糖尿病模型。将造模成功的大鼠按血糖高低随机分组,分为模型组、厄贝沙坦组、海昆小剂量组和海昆大剂量组,给药12周。第4、8、12周末收集大鼠24h尿量及检测尿蛋白量。12周末处死大鼠,取血并分离血清检测Scr、BUN和TG等,取肾后固定包埋切片,采用HE染色,Mallory染色观察肾组织病理改变,并应用免疫组化检测肾组织VEGF蛋白的表达。结果:海昆肾喜能够降低糖尿病大鼠的蛋白尿,增加体重,降低Scr和BUN,改善肾功能,调节TG,改善脂代谢紊乱,并且能够降低糖尿病大鼠肾小管上皮细胞、肾间质细胞内的VEGF在细胞浆内的表达(P<0.01)。结论:海昆肾喜可能抑制VEGF蛋白的过度表达,进而减缓糖尿病肾病的病程进展。Objective ： To study the expression of vascular endothelial growth factor（VEGF） in the renal tubules of STZ- induced diabetic nephropathy rats. Methods ：SD rats were randomly divided into two groups： normal control and diabetic nephropathy. Diabetes was induced by intraperitoneal injection of STZ. And the diabetic rats were randomly divided into four groups： model group,irbesartantreatment group, Haikun Shenxi low and high dose treatment group. 24 -- hour urine volume, 24- hour urinary protein excretion were examined at week 4, 8, and 12 respectively. All the rats were sacrificed at week 12; body weight, kidney weight,and serum excretion, serum creatinine, blood urea nitroge were improved by Haikun Shenxi preparation. The expression of VEGF in the Haikun Shenxi treatment group was depressed greatly （ P 〈 0.01 ） in the renal tubular epithelial cell and renal interstitial cell. Conclusion ： The overexpression of VEGF in renal of diabetic rats may be one of the mechanisms of diabetic nephropathy, Haikun Shenxi had certain renoprotective effects.

关 键 词：糖尿病肾病 海昆肾喜 血管内皮生长因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]