山仙颗粒对Lewis肺癌转移过程中E-cadherin CD44V6及nm23-H1表达影响的实验研究  

The Experimental Study of SXG Influences on the Expression of E-cadherin CD44V6 and nm23-H1 in Lewis Lung Cancer Metastatic Process

作　　者：庞淑珍[1] 关建民[1] 王旭[1] 吴爱华[1] 王中英[1] 刘颖[1] 

机构地区：[1]山东省菏泽医学专科学校,山东菏泽274030

出　　处：《中华中医药学刊》2008年第10期2266-2268,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：荷泽医学专科学校科研项目(H07K01)

摘　　要：目的:探讨山仙颗粒对Lewis肺癌转移过程中E-cadherin、CD44V6及nm23-H1表达的影响。方法:将45只C57BL/6小鼠随机分成空白对照组、荷瘤对照组、山仙颗粒组。给后两组接种瘤细胞,山仙颗粒组用0.5g/mL的山仙颗粒悬液0.4mL(只.天)灌胃,其他两组用生理盐水灌胃。采用免疫组化S-P法测45只小鼠肺组织、瘤组织内E-cadherin、CD44v6和nm23-H1表达情况。结果:荷瘤对照组与山仙颗粒组比较,出瘤时间短,瘤重及肺转移灶数高,差异有显著性(P<0.05);与山仙颗粒组、空白对照组比较,E-cadherin和nm23-H1的阳性表达率低,CD44V6的阳性表达率高,差异有显著性(P<0.05,P<0.01)。结论:E-cadherin、CD44V6和nm23-H1异常表达在Lewis肺癌形成和转移过程中发挥着重要作用,山仙颗粒能提高E-cadherin和nm23-H1的表达,降低CD44V6的表达。山仙颗粒有抗恶性肿瘤形成和转移的作用。Objective： To detect SXG influencing on the expression of E - cadherin, CD44V6 and nm23 - H1 in Lewis lung cancer metastatic process. Methods ： 45 mice were randomly divided into three groups： blank control groups, with tumor group and ShanXian granule（SXG） group. Inoculating the mice in the latter two group with tumor cell. The SXG group were given SXG （0.5g/mL） 0.4mL/（ one · day） , other two groups were given normal saline. Observed the time of the tumor coming into being. The expression of E - cadherin, CD44V6 and nm23 - H1 in mice lung and tumor tissue, were measured with the immunohistochemical S - P method. Results ： Tumor grouo compared with SXG grouo, the former generated tumor faster, the tumor weight were higher and more lung metastasis. There was obviously diffrence between two group（ P 〈 0.05 ）. Compared with SXG group and blank group, the positive expressive rate of E - cadherin and rim23 - H1 was lower, but the positive rate of CD44V6 was higher, there was obviously statistical significance （ P 〈 0. 05 ,P 〈0.01 ）. Conclusion：The abnormal expression of E - cadherin, CD44V6 and nm23 - H1 in the process of Lewis lung cancer form and mecastasis play an important role. SXG can enhance the expression of E - cadherin and nm23 - H1, but depress the expression of CD44V6. So SXG has function witch can resist to form and metastasis of malignant tumor.

关 键 词：山仙颗粒 免疫组织化学 E—cadherin CIM4V6 NM23-H1 

分 类 号：R285.5[医药卫生—中药学]