猪肺炎支原体PCR鉴定方法的建立  

Establishment of PCR Method for the Identification of Mycoplasma hyopneumoniae

作　　者：刘茂军[1] 李冬梅 邵国青[1] 冯志新[1] 吴叙苏[1] 甘源[1] 刘冬霞[1] 

机构地区：[1]江苏省农业科学院兽医研究所,江苏南京210014 [2]秦皇岛正大有限公司,河北山海关066200

出　　处：《金陵科技学院学报》2008年第3期99-101,共3页Journal of Jinling Institute of Technology

基　　金：江苏省自然科学基金项目(BK2007711);江苏省农业三项工程项目(sx(2007)082);江苏省农业科学院院基金项目(6510725)

摘　　要：根据猪肺炎支原体的P36基因序列设计一对引物,建立猪肺炎支原体PCR检测方法,并对该方法进行了特异性和敏感性试验。成功建立了猪肺炎支原体特异且敏感的PCR检测方法,可区别猪鼻支原体、絮状支原体和鸡毒支原体,最低检出量为4.26 ng。Aim To establish a PCR method for identification of Mycoplasma hyopneumoniae （Mhp）. Method According to the P36 gene of Mhp, two primers were designed. And the specificity and sensibility assay were performed. Result Mhp gene could be Specifically amplified and be different from the genes of Mycoplasma hyorhinitis, Mycoplasrna hyorhinitis and Mycoplasma gallisepticum. In addition, the method could detect at least 4.26ng of Mhp genome. Conclusion The PCR method for detecting Mhp was developed with high specificity and sensibility.

关 键 词：猪肺炎支原体 PCR 鉴定 

分 类 号：S852.62[农业科学—基础兽医学]