实验性精索静脉曲张对大鼠同侧睾丸的影响  

Experimental Varicocele Affects the Ipsilateral Testis in Rats

作　　者：郑轶群[1] 张孝斌[1] 程帆[1] 周本正[1] 饶婷[1] 费安华[1] 

机构地区：[1]武汉大学人民医院泌尿外科,湖北武汉430060

出　　处：《中华男科学杂志》2008年第9期805-809,共5页National Journal of Andrology

摘　　要：目的:利用大鼠动物模型来研究实验性精索静脉曲张(EV)对其同侧睾丸的影响。方法:EV模型的制作是通过在雄性SD大鼠左侧精索内静脉和肾上腺静脉内侧部分结扎左肾静脉。60只雄性青春期SD大鼠随机分为EV持续6、12、18周组(EV6、EV12、EV18组,每组12只)和相应的接受假手术的对照组(每组8只)。于6、12、18周,摘取成功复制成EV模型大鼠(分别为10,8,9只)以及各自接受假手术的对照组大鼠左侧睾丸,检测其Johnsen's评分、生精小管超微结构、睾丸组织睾酮浓度(ITC)和生精细胞的凋亡指数(AI)。结果:EV6、EV12、EV18组Johnsen's评分(6.92±0.52,4.83±0.41,2.95±0.26)和ITC(6.32±0.85,5.17±0.76,4.11±0.69)均显著低于各自对照组[(9.56±0.35,9.63±0.31,9.39±0.46)和(9.64±1.23,9.38±0.69,9.73±0.49)](P<0.05),并且随着精索静脉曲张持续时间的增加而逐渐降低;EV6、EV12、EV18组AI(5.32±1.23,15.21±0.97,21.13±1.12)显著高于各自对照组(3.21±1.15,3.43±1.21,3.61±1.15),并且随着精索静脉曲张持续时间的增加而逐渐升高;各EV组生精小管的超微结构损伤也随着精索静脉曲张持续时间的增加而逐渐严重。结论:EV导致同侧睾丸ITC下降、生精功能的进行性损害、生精细胞AI增加。Objective： To investigate the impact of experimental varicocele （EV） on the ipsilateral testis in rats. Methods： EV was induced by partial ligation of the left renal vein in male SD rats, the control rats subjected to sham operation, and the testes of the EV models and controls were extirpated 6, 12, and 18 weeks later. Johnson＇s score, uhrastrueture of seminiferous tubules, i ntratesticular testosterone concentration （ITC） and germ cell apoptotic index （ AI ） of each left testis were evaluated. Results ： Johnson＇s scores were （6.92 ±0.52）, （4.83 ±0.41） and （2.95 ±0.26）, ITCs were （6.32 ±0.85） , （5.17 ±0.76） and （4.11±0.69） and AIs were （5.32 ± 1.23）, （ 15.21±0.97） and （21.13 ± 1.12） respectively in the 6 w , 12 w and 18 w EV groups, significantly lower than in the corresponding control groups, （9.56 ± 0.35, 9.63 ± 0.31, 9.39 ± 0.46 ）, （9.64 ± 1.23, 9.38 ± 0.69, 9.73± 0.49） and （3.21 ± 1.15, 3.43 ± 1.21,3.61 ± 1.15） （P 〈0.05）, the former two showing a gradual decline while the latter a significant elevation with the increasing duration of varicoeele. The damage to the uhrastructure of seminiferous tubules was aggravated with the prolonging of varicocele. Conclusion ： EV can cause a progressive decline of ITC, dyszoospermia and increased AI of germ cells.

关 键 词：精索静脉曲张 睾酮 凋亡 大鼠 

分 类 号：R697.24[医药卫生—泌尿科学] R-332[医药卫生—外科学]