长毛对虾血细胞与血淋巴液的生化特性初步研究  

Biochemical Properties of Hemocytes and Hemolymph of Fenneropenaeus penicillatus

作　　者：贾永红[1] 裘洁圆[1] 周秋燕[1] 陈吉刚[1] 

机构地区：[1]浙江万里学院生物与环境学院,宁波315100

出　　处：《动物学杂志》2008年第5期104-109,共6页Chinese Journal of Zoology

基　　金：宁波市博士基金项目(No.2005A610026);宁波市自然科学基金项目(No.2007A610070);浙江省教育厅项目(No.20050223);浙江万里学院校项目和博士启动基金

摘　　要：长毛对虾（Fenneropenaeus penicillatus）是我国东南沿海一带一种主要的水产经济动物。为了解其血淋巴液的基本特性,借助显微观察、生化实验等方法对长毛对虾的血细胞和血浆的细胞化学特征进行了初步研究。结果表明,长毛对虾血细胞密度为（0.43±0.11）×10^7个/ml;可明显分无颗粒细胞：长径（9.7±1.4）μm、短径（5.8±0.8）μm,占细胞总数的16.2%±1.8%;大颗粒细胞：长径（11.3±1.3）μm、短径（6.7±1.7）μm,占细胞总数的46.6%±3.2%;小颗粒细胞：长径（7.1±0.4）μm、短径（5.4±0.4）μm,占细胞总数的35.7%±4.5%。在16～18℃下,60min的反应时间内,血细胞对金黄色葡萄球菌（Staphylococcusaureus）菌体细胞悬液（10^6～10^7个/ml）的吞噬率为67%±5%。长毛对虾血浆蛋白浓度为11.78mg/ml,对3%的鸡血红细胞悬液的溶血相对活性为2.6,对5%的鸡血红细胞悬液的凝集效价为15～18。血浆平板抑菌实验表明,长毛对虾血浆对金黄色葡萄球菌有明显的抗菌效果,抑菌圈大小为（10±1）mm,对大肠杆菌（Escherichia coli）的抑菌圈大小为（6±2）mm。本工作为长毛对虾血浆中诸多活性多肽的后续研究奠定了基础。Fenneropenaeus penicillatus is one of the main aquatic creatures widely disturbed in southern-east coast of Ningbo, China. To understand the cellular and chemical characteristics, the hemocytes and hemolymph of F. penicillatus were isolated and primarily studied using microscope and chemical analysis. The results showed that the density of hemocyte was （0.43 ± 0.11） × 10^7/ml. A total of three types of hemocytes were isolated including agranuler hemocyte （AH）： length 9.7 ± 1.4μm, width 5.8 ± 0.8μm, occupying 16.2% ± 1.8% of the total hemocytes; large-granule hemocyte （LGH） ： length 11.3 ± 1.3μm, width 6.7 ± 1.7μm, occupying 46.6% ± 3.2% of the total hemocytes; small-granule hemocyte （SGH）： length 7.1 ±0.4μm, width 5.4 ±0.4 μm, occupying 35.7% ±4.5% of the total hemocytes. Under the condition of 16 - 18℃ and 60 min incubation time, the phagocytic rate of hemocytes on Staphylococcus aureus （ 10^6 - 10^7/ml） was 67% ± 5% . The protein content of hemolymph of the shrimp was 11.78 mg/ml, the relative hemolysis activity of the hemolymph to 3% cock red cells was 2.6, and the coagulant value of the hemolymph to 5% cock red cells was 15 - 18. The hemolymph showed obvious antimicrobial activity by measuring the area of inhibition of growth around the disc with 10 ± 1 mm to Staphylococcus aureus and 6 ＋ 2 mm to Escherichia coli. The work laid a foundation to the further research of the active peptides.

关 键 词：长毛对虾 血细胞 血浆 

分 类 号：S917.4[农业科学—水产科学]