人类胎盘一氧化氮合成酶的组织化学定位  

Histochemical Localization of Nitric Oxide Synthase in the Human Placenta

作　　者：张中芳 林宜珍[1] 谈海英[1] 

机构地区：[1]湖南医科大学附属第二医院妇产科

出　　处：《中华妇产科杂志》1997年第11期662-665,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金

摘　　要：目的：了解一氧化氮合成酶（ＮＯＳ）在胎盘中的定位及分布，探讨一氧化氮（ＮＯ）在妊娠中的生理病理作用。方法：采用ＮＡＤＰＨ－ｄ组织化学方法，对１６例早孕绒毛、２１例正常足月妊娠胎盘、１５例重度妊高征胎盘、２例ＩＵＧＲ胎盘进行ＮＯＳ的定位研究。结果：早孕、正常足月妊娠者合体滋养细胞内有甲（ｆｏｒｍａｚａｎ）沉着，并多见于细胞基底部，细胞滋养细胞内无甲沉着，末梢绒毛中的少数毛细血管壁有甲沉着。ＩＵＧＲ、重度妊高征合体滋养细胞内亦有甲沉着，但主要位于细胞顶部，量较孕周相近的正常足月妊娠者少，末梢绒毛中的大多数毛细血管壁有甲沉着。结论：早孕、正常足月妊娠、ＩＵＧＲ、重度妊高征时，绒毛及胎盘ＮＯＳ活性部位呈特定的分布。ＩＵＧＲ、重度妊高征胎盘合体滋养细胞ＮＯＳ活性比相近孕周的正常足月妊娠者低，分布有差异。Objectives: To study localization, distribution and activity of nitric oxide synthase (NOS) in the human placenta and to speculate the action of nitric oxide (NO) during pregnancy. Methods: NADPHdiaphorasc histochemical method was used to indicate the distribution, localization and activity of NOS in the placentae of normal term pregnancy (n=21). Severe pregnancy induced hypertension (PIH) (n=15), intrauterine growth retardation (IUGR) (n=2) and villi of early pregnancy (n=16). Results: The NOS reactive product called blue formazan was found in most of syncytiotrophoblast (STr) and located in top of cells in severe PIH and IUGR placentae. In normal term placentae the blue formazan was found in STr and located in base of cells. The number of blue formazan was more than that of PIH and IUGR placentae. Endothelial cells of most capillaries of the terminal villi in sever PIH and IUGR placentae were found to be deposited with blue formazan, but in normal term pregnancy, blue formazan was found only on rare occasions. Conclusions: Distribution and localization of NOS in placentae of normal term pregnancy, severe PIH and IUGR cases and early villi are specific. The NOS activity of STr in severe PIH placentae and IUGR placentae are lower than that of normal term placentae. The distribution and localization of NOS within the STr in severe PIH placentae and IUGR placentae are different from those in normal term placentae. The low activity of NOS secreted by placenta may be relative to the pathogenesis of PIH and IUGR.

关 键 词：胎盘 组织细胞化学 一氧化氮合成酶 

分 类 号：R321－33[医药卫生—人体解剖和组织胚胎学] R362[医药卫生—基础医学]