感觉神经端侧缝合后神经再生趋化性的实验研究  

Experimental study of neurotropism following end-to-side neurorrhaphy of sensory nerve

作　　者：虞庆[1] 王涛[2] 顾玉东[2] 

机构地区：[1]温州医学院第二附属医院骨科,浙江温州325000 [2]复旦大学附属华山医院手外科,上海200040

出　　处：《温州医学院学报》2008年第5期446-448,共3页Journal of Wenzhou Medical College

基　　金：温州市科技局科研基金资助项目(Y20060214)

摘　　要：目的:探讨感觉神经端侧缝合后神经再生的趋化性问题。方法:健康雌性SD大鼠20只,分为神经端侧缝合组和正常对照组,每组10只。端侧缝合组切断前臂内侧皮神经,尺神经后内侧方外膜开窗,将前臂内侧皮神经和开窗的尺神经外膜做端侧缝合,正常对照组仅显露前臂内侧皮神经和尺神经。1年后采用乙酰胆碱酯酶染色和Fluoro-Gold荧光逆行示踪技术,观察和计数前臂内侧皮神经纤维和大鼠脊髓C5～T1前角和背根神经节神经元。结果:乙酰胆碱酯酶染色结果显示,正常组未发现运动神经纤维,端侧缝合组有髓神经纤维数目是正常对照组的23.0%(P<0.01),运动神经纤维占有髓神经纤维的比例为0.34±0.16(P<0.01)。神经元逆行荧光示踪结果显示,正常组脊髓前角未发现荧光标记阳性的神经元;端侧组脊髓前角荧光标记神经元数目为150.9±104.8(P<0.01),运动神经元比例为0.27±0.11(P<0.01)。结论:感觉神经端侧缝合后神经侧方出芽对特定靶器官的趋化性不明显。Objective： To detect whether there is neurotropism following end-to-side neurorrhaphy of sensory nerve. Methods： Twenty female Sprague-Dawley rats were divided into 2 groups： end-to-side neurorrhaphy group and normal control group. In the end-to-side group, the cutaneous antebrachli medialis nerve（CAM） was transected, then an i mm epineural window was created on the ulnar nerve. Distal end of CAM nerve was sutured to the windowed ulnar nerve by means of end-to-side neurorrhaphy. In the normal control group, CAM and ulnar nerves were exposed. One year post-operatively, evaluations including acetylcholinesterase （AchE） stain and retrograde Fluoro-Gold neuron tracing were conducted. Results： AchE staining showed that in the endto-side group myelinated nerve fiber count was 23.0% as compared to normal control group （P〈0.01）, and the percentage of motor fibers in the end-to-side group was 0.34 ± 0.16 （P〈0.01）. Retrograde neuron tracing demonstrated that the motor neuron counts in CAM end-to-side group were 150.9±104.8 （P〈0.01）, and the percentage of motor neuron in the end-to-side group was 0.27 ± 0.11 （P〈0.01）. Conclusion： Neurotropism in collateral spouting after end-to-side neurorrhaphy of sensory nerve is not significant.

关 键 词：周围神经 端侧缝合 趋化性 

分 类 号：R651.3[医药卫生—外科学]