The hybridization between peptide nucleic acid containing azobenzene and DNA labeled nanoparticle on chip surfaces studied by atomic force microscopy  

作　　者：LI JinDu CHEN Miao ZHANG HaoBo LIU Sheng LIU JianXi 

机构地区：[1]State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Chinese Science Bulletin》2008年第19期3077-3080,共4页

基　　金：Supported by "973" Project of the Ministry of Science and Technology of China (Grant No. 2007CB607605);National Natural Science Foundation of China (Grant No. 20473106)

摘　　要：Peptide nucleic acid containing azobenzene (N-PNA) was covalently bound on chip surfaces. Complementary DNA probes labeled by gold nanoparticles hybridizated with PNA on chip surfaces. The hybridization was successfully detected with AFM by the microscopy mothed. The influences of the position and the cis-trans isomerization of azobenzene unit on hybridization were investigated with AFM.Peptide nucleic acid containing azobenzene （N-PNA） was covalently bound on chip surfaces. Complementary DNA probes labeled by gold nanoparticles hybridizated with PNA on chip surfaces, The hybridization was successfully detected with AFM by the microscopy mothed. The influences of the position and the cis-trans isomerization of azobenzene unit on hybridization were investigated with AFM.

关 键 词：.缩氨酸 偶氮苯 异构化作用 显微镜 

分 类 号：O62[理学—有机化学]