运动对胰岛素抵抗大鼠脂肪组织肿瘤坏死因子-α表达的影响  

Exercise and the expression of TNF-α in the adipose tissue of rats with insulin resistance

作　　者：孙晖[1] 肖方喜[2] 陈璐璐[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科,武汉430022 [2]武汉市第一医院内分泌科

出　　处：《中华物理医学与康复杂志》2008年第9期594-597,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：湖北省科技攻关基金资助项目（2005AA30C133）

摘　　要：目的研究运动对高脂饮食诱导的胰岛索抵抗（IR）大鼠脂肪组织中肿瘤坏死因子-α（TNF-α）表达的影响。方法将30只Wistar大鼠随机分为对照组和高脂组，分别给予基础饲料与高脂饲料喂养18周；将造模成功的高脂组IR大鼠随机分为静息组和运动组，均继续给予高脂饲料喂养，同时对运动组大鼠实施游泳训练，共持续6周。于实验进行24周时检测各组大鼠体重、血清空腹血糖（FBG）、空腹胰岛素（FINS）和游离脂肪酸（FFA）水平，并同时计算胰岛素敏感指数（ISI）；采用实时荧光定量聚合酶链反应、免疫印迹技术分别检测各组大鼠脂肪组织中TNF-α mRNA及蛋白表达水平。结果喂养18周时发现高脂组ISI较对照组明显降低，提示高脂组IR模型制作成功；运动组大鼠经游泳训练6周后，其ISI较静息组明显升高（P〈0．05），但仍显著低于对照组水平（P〈0．01）；静息组大鼠脂肪组织中TNF-αmRNA及蛋白表达水平均较对照组明显升高（P〈0．05）；运动组大鼠脂肪组织中TNF-αmRNA和蛋白表达较静息组进一步升高（P〈0．05）。结论高脂喂养可诱导大鼠产生IR，规律运动可减轻大鼠IR状态，其机制可能与上调脂肪组织中TNF-α表达有关。Objective To observe the effect of exercise on the expression of TNF-αin the adipose tissue of insulin resistant rats fed a high fat diet. Methods Thirty healthy male rats were randomly divided into a high fat diet group and a normal chow group. Eighteen weeks later, the high fat group was randomly divided into a resting group fed with the high fat diet only, and an exercise group fed the high fat diet, but receiving swimming training for 6 weeks. Changes in their metabolism of glucose and lipids were observed, and the insulin sensitivity index was calculated. Meanwhile, the level of TNF-α mRNA in their adipose tissue was detected with a real-time fluorescence quantitative polymerase chain reaction （PCR） , and protein in the adipose tissue was measured using Western blotting. Results After 18 weeks of high fat diet feeding, the insulin sensitivity index of the high fat diet group decreased significantly as compared to the normal chow group, suggesting that insulin resistance had been acquired in the .high fat diet group. 24 weeks later, the insulin sensitivity index of the resting group had decreased further, again significantly when compared to the normal chow group. Compared to the resting group, the insulin sensitivity index of exercise group was significantly higher, and the expression of TNF-α mRNA and protein in their adipose tissue was significantly increased. Conclusion Insulin resistance can be induced by high fat diet feeding. Exercise can improve insulin resistance by increasing the expression of TNF-α in adipose tissue.

关 键 词：运动 胰岛素抵抗 肿瘤坏死因子-Α 

分 类 号：R587.1[医药卫生—内分泌]