MiniFiler试剂盒进行微量细胞STR分型可行性探讨  

The exploration of STR genotyping of minute cells by MiniFiler kit

作　　者：苏芹[1] 严红[2] 李彩霞[2] 于子辉[1] 胡兰[2] 

机构地区：[1]中国人民公安大学,北京100038 [2]公安部物证鉴定中心,北京100038

出　　处：《中国法医学杂志》2008年第5期330-332,共3页Chinese Journal of Forensic Medicine

基　　金：国家"十一五"科技支撑计划项目(2006BAK07B01)

摘　　要：目的探讨采用MiniFiler试剂盒进行微量细胞STR分型的可行性。方法利用显微操作法捕获微量口腔上皮细胞,采用MiniFiler试剂盒进行STR复合扩增,ABI 3130遗传分析仪检测STR分型。结果10个口腔上皮细胞能够获得稳定的STR分型;1、3、5个口腔上皮细胞能够获得完整的STR分型,但存在随机性。结论微量细胞进行STR分型具有不稳定性,目前还难以在实际检案中应用,但可以通过增加模板量来提高分型的成功率。Objective Explore the feasibility of minute ceils STR typing. Methods Minute cells which were obtained by micromanipulation method were amplified with MiniFiler kit, and detected by ABI 3130 genetic analyzer. Results Ten cells can be successfully genotyped. Complete genotyping results can be obtained for one, three or five cells but with stochastic effect. Conclusion Instability is observed in minute cells genotyping, so it can not be used in actual case work. Maybe increase the quantity of DNA template can improve the success rate.

关 键 词：法医物证学 显微操作法 MiniFiler试剂盒 口腔上皮细胞 STR 

分 类 号：D919[医药卫生—法医学]