Isolation and identification of Sclerotinia stem rot causal pathogen in Arabidopsis thaliana  

作　　者：Ai-rong WANG Wen-wei LIN Xiao-ting CHEN Guo-dong LU Jie ZHOU Zong-hua WANG 

机构地区：[1]Computer and Information College, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2]Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2008年第10期818-822,共5页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：the National Natural Science Foundation of China (No. 30471178);the Ministry of Agriculture of China (No.nyhyzx07-054)

摘　　要：A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fungal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoculation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum.A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fungal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoculation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum （Lib.） de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA （LSU rDNA） by polymerase chain reaction （PCR）, and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum （Lib.） de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum.

关 键 词：Sclerotinia sclerotiorum Arabidopsis thaliana Large subunit ribosomal DNA （LSU rDNA） Systematic classification 

分 类 号：Q949.32[生物学—植物学]