益气清热方及其拆方的兔含药血清对Ⅰ型幽门螺杆菌致GES-1细胞凋亡的影响  

Effects of rabbit serum containing Yiqi Qingre Formula and its decomposed recipes on type Ⅰ Helicobacter pyloriinduced apoptosis of GES-1 cells in vitro

作　　者：陆为民[1] 沈洪[1] 严士海[2] 杨学文[3] 朱萱萱[2] 缪界平[3] 单兆伟[1] 

机构地区：[1]江苏省中医院消化科,江苏省南京市210029 [2]江苏省中医院药理研究室,江苏省南京市210029 [3]江苏省中医院检验科,江苏省南京市210029

出　　处：《世界华人消化杂志》2008年第27期3026-3030,共5页World Chinese Journal of Digestology

基　　金：江苏省中医管理局课题资助项目;No.200304~~

摘　　要：目的:观察益气清热方及其拆方对Ⅰ型Hpylori致GES-1细胞凋亡的影响,探讨益气清热法治疗Hpylori感染的机制.方法:按生理盐水、阿莫西林、益气清热方、黄芪、黄芩分组,制备兔含药血清.建立Ⅰ型Hpylori致GES-1细胞病变的模型,分别加入100mL/L各组兔含药血清的完全培养液,继续培养48h,收集与固定细胞,用流式细胞仪和Hoechst33258荧光染料检测细胞凋亡情况.结果:经Ⅰ型Hpylori感染后,GES-1细胞凋亡率显著增加,阿莫西林、黄芪、黄芩及益气清热方均能降低其凋亡率(1.3633±0.4229,2.1925±0.6779,1.7967±0.6987,1.4740±0.4156vs16.6229±8.5087,P<0.01或0.05),Hoechst33258荧光染料的检测结果与流式细胞仪检测结果有较好的一致性.结论:益气清热方可能通过抑制细胞的凋亡,减轻Hpylori对胃上皮细胞的损伤,维持细胞的正常生长,达到治疗目的.AIM： To observe the effects of rabbit serum containing Qi-replenishing heat-clearing formula （Yiqi Qingre Formula） and its decomposed recipes on type ⅠHelicobacter pylori （H pylori） -induced GES-1 cell apoptosis and to explore its mechanism. METHODS： Rabbit sera saline, containing amoxicillin, Yiqi Qingre Formula, Huangqi or Huangqin were prepared. The model of type Ⅰ H pylori-induced GES-1 cell injury was es-tablished and co-cultured with the individu-ally complete culture fluid of 100 mL/L rabbit serum for 48 h. Then, cells were collected and fixed, followed by identification of apoptosis using flow cytometry and Hoechst33258 fluo-rescent staining. RESULTS： Apoptosis rate of type ⅠH pylori-infected GES-1 cells remarkedly increased in the saline group, while it was decreased either by amoxicillin, Yiqi Qingre Formula, Huangqi or Huangqin （1.3633 ± 0.4229, 2.1925 ± 0.6779, 1.7967 ± 0.6987, 1.4740 ± 0.4156 vs 16.6229 ± 8.5087, P 〈 0.01 or 0.05）. The coincidence rate determined using flow cytometry and Hoechst33258 fluores-cent staining was high. CONCLUSION： Yiqi Qingre Formula has the effects of alleviating type ⅠH pylori-induced gastric epithelial cell injury through inhibition of cell apoptosis, thus maintaining cell growth.

关 键 词：益气清热方 Ⅰ型幽门螺杆菌 人胃上皮细胞系GES-1 中药 血清药理学 细胞培养 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]