蛙皮素对内毒素致肠黏膜损伤的保护作用  

Protective effects of bombesin on endotoxin-induced intestinal injury in rats

作　　者：龚俊[1] 朱尤庆[1] 

机构地区：[1]武汉大学中南医院消化内科,湖北省武汉市430071

出　　处：《世界华人消化杂志》2008年第27期3050-3054,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨蛙皮素对内毒素致肠黏膜损伤的影响.方法:大鼠96只随机分成3组:A生理盐水组(ip生理盐水1mL/kg),B内毒素组(ipLPS5mg/kg),C蛙皮素组(实验前1wk每天sc蛙皮素10μg/kg,每天3次,至第7天ipLPS5mg/kg).在注射LPS2,6,24h后,各组处死大鼠8只,留取肠组织.光镜下观察肠组织病理形态学改变,生化法检测肠组织匀浆丙二醛(MDA)和谷胱苷肽过氧化物酶(GSH-PX)的含量,采腹腔静脉血,双抗夹心法(ELISA)检测血清IL-6和IL-10.结果:肉眼和光镜可见C组肠黏膜损伤较B组明显减轻.C组MDA含量在2、6、24h时间点均较B组降低(3.4092±0.7765vs8.4359±0.5589;4.6588±0.3311vs10.9375±1.00094;4.6324±0.2278vs10.5074±1.0142,均P<0.01),但仍较A组高;C组GSH-PX活力单位较B组显著升高(2h:1231.83±74.68vs766.67±57.94;6h:2113.34±81.42vs749.09±56.72;24h:1950.48±72.74vs785.62±62.43,均P<0.01);C组IL-6水平于2、6h较B组降低(2h:553.70±23.03vs652.81±27.09;6h:494.52±40.68vs606.68±43.60;24h:571.56±52.54vs602.73±25.19,均P<0.01),但仍高于A组;C组IL-10水平于各时间点均较B组升高(2h:601.58±30.65vs478.76±23.49;6h:653.77±35.75vs469.35±11.27;24h:714.04±29.55vs635.17±29.15,P<0.01).结论:蛙皮素具有抗氧化损伤及抗炎症反应的作用.AIM： To investigate the protective effects of bombesin on endotoxin-induced intestinal injury in SD rats. METHODS： A total of 96 SD rats were divided randomly into 3 groups. Group A was served as control group and administrated normal sa-line （1 mL/kg）. Group B was the model group and treated with LPS （intraperitoneally, 5 mg/kg）. Rats in Group C were firstly treated with bombesin （subcutaneously, 10 μg/kg, three times a day） for 7 d, and then injected with LPS intraperitoneally. Following LPS administration, intestinal tissues were collected at 2, 6, 24 h sepa-rately for Malondialdehyde （MDA） and GSH-PX study, and blood samples were collected for IL-6 and IL-10 study. RESULTS： According to the macroscopic and microscopic findings, group C showed signifi-cantly lower damage and inflammation com-pared with group B. At 2, 6, 24 h, the intestinal MDA levels were significantly lower in group C than in group B （3.4092 ± 0.7765 vs 8.4359 ± 0.5589; 4.6588 ± 0.3311 vs 10.9375 ± 1.00094; 4.6324 ± 0.2278 vs 10.5074 ± 1.0142, all P 〈 0.01）, but still higher than that in group A. The intestinal GSH-PX levels were significantly higher in group C than in group B （2 h： 1231.83 ± 74.68 vs 766.67± 57.94; 6 h： 2113.34 ± 81.42 vs 749.09 ± 56.72; 24 h： 1950.48 ± 72.74 vs 785.62 ± 62.43, all P 〈 0.01）. At 2, 24 h, the IL-6 levels were lower in group C than in group B （2 h： 553.70 ± 23.03 vs 652.81 ± 27.09; 6 h： 494.52 ± 40.68 vs 606.68 ± 43.60; 24 h： 571.56 ± 52.54 vs 602.73 ± 25.19, all P 〈 0.01）, but still higher than in group A. At every time, the IL-10 levels were higher in group C than in group B （2 h： 601.58 ± 30.65, vs 478.76 ± 23.49; 6 h： 653.77 ± 35.75 vs 469.35 ± 11.27; 24 h： 714.04 ± 29.55 vs 635.17 ± 29.15, all P 〈 0.01）. CONCLUSION： Bombesin has the function of anti-oxidation and anti-inflammation by increas-ing the levels of GSH-PX and IL-10 and decreas-ing the levels of MDA and IL-6.

关 键 词：内毒素 蛙皮素 丙二醛 谷胱苷肽过氧化物酶 白介素-6 白介素-10 

分 类 号：R285.5[医药卫生—中药学]