以肝素固化脱细胞支架构建的小口径抗凝人工血管  

Constructing small-caliber anticoagulant vascular grafts using decellularized scaffolds with heparin immobilization

作　　者：周敏[1] 刘长建[1] 卫志庆[1] 乔威[1] 乔彤[1] 冉峰[1] 张明[1] 

机构地区：[1]南京大学医学院附属鼓楼医院血管外科,江苏省南京市210008

出　　处：《中国组织工程研究与临床康复》2008年第36期7118-7121,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:目前临床上直径<6mm的小口径人工血管因生物相容性差、远期通畅率低,使用效果并不理想。目的:通过对脱细胞血管支架表面固化肝素,制备一种具有抗凝血功能的小口径生物人工血管。设计、时间及地点:细胞学、组织病理学体外观察,于2005-12/2007-12在鼓楼医院实验室完成。材料:普通成年杂种犬8只,雌雄不限,体质量约20kg。方法:采用去污剂-酶消化法制备犬颈动脉脱细胞支架,并将肝素分子交联至支架表面。将制备的肝素固化血管和脱细胞血管基质植入犬颈动脉进行异体血管移植实验。主要观察指标:通过血小板黏附实验和细胞接触实验评价其血液、生物相容性能;经异体血管移植实验观察其早期血栓形成情况。结果:脱细胞支架细胞成分去除完全,而胞外基质保留完整;经肝素固化后具有良好的抗凝血性能,细胞接触实验未显示细胞毒性;植入异体犬颈动脉1个月后,发现肝素固化组移植血管均维持通畅,而植入单纯脱细胞血管基质者因血栓形成而闭塞。结论:对同种异体脱细胞血管支架进行表面肝素固化,可获得一种具有良好生物相容性和血液相容性的小口径人工血管。BACKGROUND： The small-caliber vascular grafts with diameter less than 6 mm can not achieve good effect for its poor biocompatibility and low graft patency. OBJECTIVE： To design a novel small-caliber vascular graft through beparin immobilization to the decellularized vascular scaffolds. DESIGN. TIME AND SETTING： The in vitro histopathology observation was performed in the Laboratory of Drum Tower Hospital from September 2005 to September 2007. MATERIALS： Six adult mongrel dogs, weighing 20 kg and irrespective of genders,were adopted. METHODS： The decellularized canine common carotid arteries were treated by detergent enzyme extraction, and covalently linked with heparin. Heparin-treated grafts and acellular matrix were implanted to carotid artery for allograft vasotransplantation experiment. MAIN OUTCOME MEASURES： Biocompatibility and blood compatibility of vascular grafts were evaluated by platelet adhesion test and cytotoxicity experiment, and the early thrombosis was assessed by allograft vasotransplantation experiment. RESULTS： Cellular components was completely removed and the extracellular matrix structure was intact remained. The decellularized-heparin-treated grafts has excellent antithrombogeneity and no cytotoxicity. Decellularized-beparin-treated grafts remained patent 1 month after implantation, while the non-heparin immobilized decellularized grafts were occluded as thrombosis. CONCLUSION： Decellularized scaffolds with heparin immobilization can be used to construct small caliber vascular grafts. The decellularized -heparin-treated grafts has excellent biocompatibility and blood compatibility.

关 键 词：脱细胞支架 肝素固化 人工血管 抗凝 生物材料 

分 类 号：R318[医药卫生—生物医学工程]