聚乙烯醇、聚乙烯吡咯烷酮和牛血清白蛋白对牛卵母细胞成熟及体细胞克隆胚胎发育的影响  

The effect of PVA,PVP and BSA for oocytes maturation and somatic cloned embryos development in vitro

作　　者：毛晶[1] 李钟淑[1] 蔡文莲[1] 胡艳明[1] 祖晶[1] 王士勇[1] 方南洙[1] 

机构地区：[1]延边大学农学院动物遗传育种与繁殖实验室,吉林龙井133400

出　　处：《黑龙江畜牧兽医》2008年第10期12-15,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：振兴东北老工业基地项目;延边大学与延边州合作项目

摘　　要：为了比较不同浓度的聚乙烯醇(PVA)、聚乙烯吡咯烷酮(PVP)、牛血清白蛋白(BSA)对牛卵母细胞体外成熟率、重组胚卵裂率和发育率的影响,试验采用抽吸法回收卵母细胞,用不同的无血清成熟液进行卵母细胞的体外成熟培养,然后对成熟卵母细胞进行核移植、融合、激活以及胚胎的体外培养。结果表明,适合延边黄牛卵母细胞成熟及体细胞克隆胚胎发育的PVA、PVP和BSA的最佳浓度分别是1.0mg/mL、0.3%和0.4%。The serum - free medium was used for oocyte maturation embryo culture and the development of culture in vitro. The ovaries were brought from slaughter house, cuted the epactal tissue and washed with 38℃ sodium chloride, recovered the oocytes, activated and cultured in vitro. The rate of maturation in vitro, the different cattle oocytes, cleavage rate and morulae blastocyst rate, were compared by different concentration of PVA, PVP, BSA. The result that the best concentration of PVA,PVP and BSA for Yan Bian Yellow Cattle is 1.0 mg/mL,0.3% and 0.4% ,respectively.

关 键 词：无血清卵母细胞成熟液 体细胞克隆 成熟率 卵裂率 

分 类 号：S814[农业科学—畜牧学]