润燥护鼻乳膏质量标准研究  

Study on Quality Standard for Runzao Hubi Cream

作　　者：陈凌云[1] 杨世珍[1] 冷秀梅[1] 郭兆刚[1] 

机构地区：[1]云南中医学院中药学院,云南昆明650200

出　　处：《云南中医学院学报》2008年第5期17-22,共6页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：云南省教育厅科学研究基金(No:5Y580483C)

摘　　要：目的:建立润燥护鼻乳膏质量标准。方法:定性鉴别采用薄层色谱(TLC)法,含量测定采用高效液相色谱(HPLC)法。结果:该制剂中的大黄、紫草、当归可用TLC法检出,且空白无干扰;含量测定的色谱条件:十八烷基硅烷键合硅胶为填充剂(4.6mm×150mm,5μm),以甲醇-0.2%磷酸(75∶25)为流动相;流速1.0mL/min,柱温30℃,检测波长254nm,理论板数按大黄素峰计算不低于3000。结论:薄层鉴别和含量测定方法操作简便,结果准确,重现性好,可用于控制润燥护鼻乳膏的质量。Objective： To establish the quality standard for Runzao Hubi Cream. Method： TLC was employed for the chemical identification, and HPLC was employed for the content determination. Result： Rheum offieinale Baill, Arnebiae uehroma （Royle） Johnst, and Angelica sinensis （Oliv） Diels could be identified by TLC. Emodin and Chrysophanol could be determined by HPLC, ODS- C18 as stationary phase, methanol- 0. 2% phosphoicaid （75：25 ） as mobile phase, flow rate was lml/min, detected wavelength at 254nm, theoretical plate number of Emodin ≥3 000. Conclusion： The method is simple, accurate and reliable, and can be used for the quality control of Runzao Hubi Cream.

关 键 词：润燥护鼻乳膏 薄层鉴别 含量测定 

分 类 号：R282.5[医药卫生—中药学]