大豆品种RGA分析与疫霉根腐病抗性鉴定  

Resistance Identification and Genetic Diversity among Soybean Cultivars Based on Resistance Gene Analogue

作　　者：孙石[1] 赵晋铭[1] 武晓玲[1] 郭娜[1] 王源超[2] 唐卿华[2] 盖钧镒[1] 邢邯[1] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室 [2]南京农业大学农业部病虫监测与治理重点开放实验室,江苏南京210095

出　　处：《作物学报》2008年第10期1704-1711,共8页Acta Agronomica Sinica

基　　金：农业部行业专项(nyhyzx07-053);教育部长江学者和创新团队发展计划(PCSIRT)

摘　　要：采用7个具有不同毒性基因的大豆疫霉菌株,对黄淮地区48个优良大豆种质资源进行了苗期接种鉴定,筛选出一批具有不同抗性的优异抗源,说明黄淮地区蕴藏着丰富的大豆抗病资源。以相似系数0.682聚类,48个大豆品种可以分成8类。同时,根据抗病基因在保守区域序列同源性的原理,利用RGA-PCR方法对48个品种的遗传多样性进行分析,从48个大豆品种的抗病基因同源序列中共扩增出53条谱带,各品种之间谱带较清晰且呈现明显的多态性,以相似系数0.746聚类,48个大豆品种可以分成7类。尽管抗性表型和RGA聚类的类与类之间没有一一对应关系,但抗谱广的品种,能较好地聚在一类,如丰收黄、科丰36、即墨油豆等。因此,综合利用抗性表型和RGA分析可以为大豆疫霉根腐病抗性基因鉴定、品种的培育和合理布局提供一定的理论依据。Phytophthora root rot caused by Phytophthora sojae is a destructive disease for soybean [Glycine max （L.） Merr.] in soybean production regions of the world. Utilization of resistant varieties is the most economical and environmentally safe method for controlling this disease. A total of 48 soybean cultivars, which mainly used in Huang-Huai Valley, were analyzed to study their resistance to P sojae by identifying seedlings with 7 stains of P. sojae. The resistance to P sojae varied among different cultivars, which were divided into 8 groups at 0.682 similarity coefficient. The results showed that there exist the rich resistant soybean resources to P. sojae in Huang-Huai Valley. Most known plant disease-resistance （R） genes include nucleotide binding site （NBS） or leucine-rich repeats （LRRs） and serine/threonine protein kinase （STK） in their encoded products domains. Two primers, XLRRfor/XLRRrev and Pto-kinl/Pto-kin2, were designed on the loci of these conserved domains. By polymerase chain reaction （PCR） and denatured polyacrylamide-gel electrophoresis techniques, disease resistance gene analogues （RGA） amplified on 48 soybean materials. The RGA analysis of 48 cultivars produced 53 amplification bands, 39 of them （73.6 %） showed polymorphic, and they were divided into 7 groups at 0.746 similarity coefficient. Although there was no parallelism relationship in groups between two different types of the clustering, the cultivars with broad resistance spectrum, such as Fengshouhuang, Kefeng 36, and Jimoyoudou could be clustered into the same group. The result indicated RGAP （resistance gene analog polymorphism） technique, combining the resistant spectrum, provides a useful and efficient way to improve the efficiency of parent selection in soybean breeding and to accelerate the process of developing soybean cultivars with resistance.

关 键 词：大豆 抗病基因同源序列分析 多态性 抗性鉴定 

分 类 号：S565.1[农业科学—作物学]