两种亲和层析法纯化白桂木凝集素的比较  

COMPARISON BETWEEN TWO AFFINITY CHROMA TOGRAPHIES IN THE PURIFICATION OF AR TOCARPUS HYPARGYREUS LECTIN

作　　者：周素芳[1] 周德义[1] 吴耀生[1] 邓勇[1] 曾麒燕[1] 

机构地区：[1]广西医科大学生物化学教研室

出　　处：《广西医科大学学报》1997年第3期17-19,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金

摘　　要：分别采用人血清γ球蛋白－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析及半乳糖（Ｇａｌ）－Ｓｅｐｈａｒｏｓｅ６Ｂ亲和层析来分离纯化白桂木凝集素（ＡＨＬ）。结果：两种方法提取的ＡＨＬ在纯度、凝集活力及回收率上均无明显差别。但用Ｇａｌ－Ｓｅｐｈａｒｏｓｅ６Ｂ来分离纯化ＡＨＬ更安全、简便、快捷和经济等优点，不失为分离桂木凝集素的一种好方法。Artocarpus hypargyreus lectin(AHL) were purified by affinity chromatography on human serum γ golubin sepharose 4B and on Gal sepharose 6B respectively The results showed that there were no significant differences as regards the purity,hemagglutinating activity and recovery rate of AHLs purified by these two methods But Gal sepharose 6B affinity chromatography is safer simpler,quicker and cheaper than human serum γ globulin sepharose 4B Thus Gal sepharose 6B affinity chromatagraphy is a good method for the purification of AHL

关 键 词：亲和层析法 白桂木凝集素 纯化 凝集素 

分 类 号：R446.62[医药卫生—诊断学] R392－33[医药卫生—临床医学]