H2-受体拮抗剂的流动注射-化学发光分析方法的研究  

Determination of H_2-receptor antagonists using flow-injection chemiluminescence method

作　　者：木尼热·阿布都克热木[1] 唐玉海[2] 木合塔尔·吐尔洪[1] 王楠楠[3] 

机构地区：[1]新疆喀什师范学院生命与环境科学系,喀什844007 [2]西安交通大学理学院分析科学研究所,西安710061 [3]内蒙古医学院第二附属医院药剂科,呼和浩特010030

出　　处：《分析试验室》2008年第10期5-9,共5页Chinese Journal of Analysis Laboratory

基　　金：新疆维吾尔自治区高等学校基金(XJEDU2006154)项目资助

摘　　要：碱性介质中K3Fe（CN）6与H2-受体拮抗剂发生氧化-还原反应产生化学发光,罗丹明6G可显著增强其发光信号,且强度与药物浓度呈良好的线性关系。据此,建立了采用K3Fe（CN）6-罗丹明6G体系测定制剂中H2-受体拮抗剂的流动注射-化学发光分析方法。在优化的实验条件下,西咪替丁在1.0×10^-9-7.0×10^-5g/mL范围内呈良好的线性关系,检出限为8.6×10^-10g/mL;盐酸雷尼替丁在1.0×10^-9-5.0×10^-5g/mL范围内呈线性关系,检出限为8.7×10^-10g/mL;法莫替丁在5.0×10^-9-7.0×10^-5g/mL范围内呈线性关系,检出限为2.4×10^-9g/mL。通过对浓度为1.0×10^-6g/mL的3种H2-受体拮抗剂分别进行的11次平行测定,其RSD分别为4.0%,3.8%和2.9%。The redoxreaction of K3 Fe（CN） 6 and H2-receptor antagonists in NaOH medium could generate weak CL signal. Rhodamine 6G could obviously sensitize this sign＇d, and the CL intensity was proportional to the concentration of H2-receptor antagonists. As a result, a new FI-CL method for the determination of H2-receptor antagonists, using the system of K3 Fe（CN）6-Rhodamine 6G has been described in this article. Under the optimal conditions, the determination range of cimetidine is 1.0 ×10^-9 - 7.0 ×10^-5 g/mE, with a determination limit of 8.56× 10^-10 g/mE; for ranitidine hydrochloride, the determination range is 1.0 × 10^-9 - 5.0×10^-5 g/mE with a determination limit of 8.69 × 10^-10 g/mL; for famotidine, the determination range is 5.0 × 10-9 - 7.0 ×10^-5 g/mE, with a determination limit of 2.35 ×10^-9 g/mE. During 11 replicate measurements for 1.0 × 10^-6 g/mE H2-receptor antagonists, the relative standard deviations are 4.0% for cimetidine; 3.8% for ranitidine and 2.9% for famotidine. This method can be successfully used to determine the content of famotidine in pharmaceutical preparations.

关 键 词：流动注射 化学发光 K3Fe(CN)6 罗丹明6G H2-受体拮抗剂 

分 类 号：O657.3[理学—分析化学]