氯酚红分光光度法测定盐酸吡格列酮  

Spectrophotometric method for determination of pioglitazone hydrochloride with eosin Y

作　　者：秦宗会[1] 沈光明 刘於生[1] 罗华生[1] 

机构地区：[1]长江师范学院化学与环境科学系,涪陵408003 [2]重庆涪陵镇安大柏中学,涪陵408103

出　　处：《分析试验室》2008年第10期15-18,共4页Chinese Journal of Analysis Laboratory

基　　金：重庆市教委科学技术(KJ081301);重庆市涪陵区科委科技计划(2008-43);第二批“重庆市高等学校优秀中青年骨干教师资助计划”[渝教人(2005)2号];长江师范学院科学技术(07Jkg50)项目资助

摘　　要：在pH9.40的Britton-Robinson广泛缓冲溶液中,氯酚红与盐酸吡格列酮反应,形成离子缔合物,其最大褪色波长位于570 nm处,最大增色波长位于434 nm处。盐酸吡格列酮的浓度分别在1.3×10^-6-1.1×10^-5mol/L和1.5×10^-6-1.2×10^-5mol/L范围内遵守比耳定律,表观摩尔吸光系数分别为4.29×10^4L.mol^-1.cm^-1和1.47×104L.mol^-1.cm^-1,检出限分别为3.50×10^-7mol/L和6.54×10^-7mol/L,回收率为100.8%-101.4%（血样）、99.3%-99.8%（混合样）、98.1%-98.6%（尿样）。方法已用于血样、尿样和混合样中盐酸吡格列酮测定。In Britton-Robinson bufer medium with pH 9.40, chlorophenol red reacts with pioglitazone hydrolehloride, to form ion-association complexes. The maximum fading color wavelength of both complexes is at 570 nm, the maximum hyperchromic wavelength locates at 434 nm. The concentration of pioglitazone hydrolchloride in the range of 1.3 × 10^-6 - 1.1 × 10^-5 mol/L and 1.5 × 10^-6 - 1.2 ×10^-5 mol/L respectively obeys beer＇s law at the maximum fading color wavelength. The apparent molar absorptivities are 4.29 × 10^4 L· mol^- 1· cm^- 1 and 1.74 × 10^4 L·mol^- 1· cm^- l, respectively. The detection limits are 3.50 ×10^-7 mol/L and 6.54×10^-7 mol/L respectively and the average recoveries 100.8% - 101.4% （blood sample）, 99.3% - 99.8% （mixture sample） and 98.1% - 98.6% （urine sampies）. The method has high sensitivity and fairly good selectivity. The method is applied to the detemaination of pioglitazone hydrolchloride in urine samples, mixture samples and blood samples, with satisfactory results.

关 键 词：氯酚红 盐酸吡格列酮 分光光度法 

分 类 号：O657.3[理学—分析化学]