酶催化动力学光度法测定H2O2  

Enzyme catalytic spectrophotometric determination of hydrogen peroxide

作　　者：陈亚红[1] 卜彦林[2] 田丰收[1] 武秋花[1] 

机构地区：[1]周口师范学院化学系,周口466000 [2]河南省水利水电学校,周口466001

出　　处：《分析试验室》2008年第10期34-37,共4页Chinese Journal of Analysis Laboratory

基　　金：河南省教育厅自然科学基金(2007150048,2008A150029)项目资助

摘　　要：H2O2能够氧化偶氮染料刚果红使之褪色,而模拟酶—血红蛋白对其具有催化作用。据此建立了一种以刚果红为指示剂的H2O2-刚果红-血红蛋白酶催化反应体系测定痕量H2O2的新方法。确定了反应的最佳条件,体系的酸度为pH10.7的NH3-NH4Cl缓冲溶液,最大吸收波长为497 nm。该法的线性范围为8.0×10^-8-8.0×10^-5mol/L,检出限为1.97×10^-9mol/L,表观摩尔吸光系数为4.6×10^4L.mol-1.cm^-1。该方法可用于雨水及消毒水中H2O2的测定。A new enzyme catalytic method was developed for the determination of trace hydrogen peroxide using hemoglobin as a catalyst. Azo dye congo red is oxidized to fade by hydrogen peroxide because of producing hydroxyl radicals in alkaline medium, which is enhanced by hemoglobin. The optimum conditions and effect of foreign ions were dis- cussed. At pH 10.7 of NH3-NH4Cl buffer solution, the system exhibited maximum absorption wavelength at 497 nm. Under the optimal conditions, the degree of fading （AA） is linear to the H2O2 concentration （C） in the range of 8.0 ×10^-8 - 8.0 × 10^-5 mol/L, and the apparent molar absorptivity E497 was 4.6 × 10^4 L·mol^-1 ·cm^-1 with the detection limit of 1.97 ×10^-9 mol/L. This method is simple, sensitive and suitable for the determination of hydrogen peroxide in disinfectors with satisfactory results.

关 键 词：催化光度法 H2O2 血红蛋白 刚果红 

分 类 号：O657.32[理学—分析化学]