美洲商陆组培快速繁殖  

Tissue Culture and Rapid Propagation of Phytolacca americana

作　　者：邹利娟[1] 苏智先[2] 胡进耀[2] 余阿梅[1] 王筱[1] 

机构地区：[1]西华师范大学生命科学学院,四川南充637002 [2]四川省生态与环境技术重点实验室,四川绵阳621000

出　　处：《中药材》2008年第9期1299-1301,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(30670209);四川省教育厅重大培育项目(07ZZ015)

摘　　要：目的:探索美洲商陆组培快速繁殖技术。方法:以无菌苗作外植体。结果:初代培养的较粗壮茎段为快速繁殖的最佳外植体,初代培养基为MS+NAA0.2 mg/L+6-BA1.0 mg/L;丛生芽最佳增殖培养基为MS+NAA0.2mg/L+6-BA2.0 mg/L,最适生根培养基为1/2MS+NAA0.4 mg/L。结论:采用美洲商陆无菌苗茎段诱导丛生芽可提高繁殖系数,为大量种植提供种苗。Objective： To explore the technique of rapid propagation for Phytolacca americana. Methods： Aseptic seedling were used as explants. Results：The best explants were the stems from strong aseptic seedling. The optimal culture media were MS ＋ NAA （0. 2 mg/L） ＋6-BA（ 1. 0 mg/L） for primarily culture, MS ＋ NAA（0. 2 mg/L） ＋6-BA（2. 0 mg/L） for the induction of clustered shoots 1/2MS with NAA 0. 4 mg/L for rooting. Conclusion ：The propagating coefficient of Phytolacca americana can be improved by inducing the clustered shoots from aseptic seedling.

关 键 词：美洲商陆 诱导丛生芽 快速繁殖 

分 类 号：R282.2[医药卫生—中药学]