五指毛桃水提物对二甲基甲酰胺所致小鼠急性肝损伤的保护作用  

The Hepatoprotective Effect of Aqueous Extracts from Ficus hirta on N,N-Dimethylformamide Induced Acute Liver Injury in Mice

作　　者：吕颖坚[1] 贾凤兰[1] 阮明[1] 张宝旭[1] 

机构地区：[1]北京大学公共卫生学院毒理系,北京100083

出　　处：《中药材》2008年第9期1364-1368,共5页Journal of Chinese Medicinal Materials

基　　金：北京市自然科学基金资助项目(7072042)

摘　　要：目的:探讨五指毛桃水提物对二甲基甲酰胺(DMF)所致小鼠急性肝损伤的保护作用。方法:雌性C57BL/6小鼠25只,雄性ICR小鼠35只,各随机分成5组:正常对照组、DMF组、五指毛桃低、中、高剂量组。五指毛桃各组每天分别灌胃给予五指毛桃水提物100、200、300 g生药/kg,共5 d,第4 d给药30 m in后,除正常对照组外各组小鼠腹腔注射DMF造模。染毒48 h后,测定血清中丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)和乳酸脱氢酶(LDH)的活性,并以光镜观察肝脏组织病理变化。结果:C57BL/6和ICR小鼠各剂量组血清中ALT、AST和LDH活性与模型组比较显著降低,肝脏组织病理损伤明显减轻。结论:五指毛桃对DMF引起的小鼠急性肝损伤具有明显的保护作用。Objective： To investigate the effect of aqueous extract from Ficus hirta on N,N-Dimethylformamide（DMF） induced liver injury in mice. Methods： C57BL/6 mice and ICR mice were randomly divided into 5 groups： negative control group, positive control group and three treated groups respectively. Treated groups were administered orally with 100,200, 300 g/kg Ficus hirta aqueous extract per day respectively for 5 days. On the 4th day, 2. 3 g/kg DMF was given by intraperitoneal injection to all C57BL/6 mice except negative control group, while for ICR, DMF was administration at a 2. 75 g/kg dose. 48h after DMF injection, serum samples were collected to determine the activities of ALT, AST and LDH and the pathological changes of liver tissue were analyzed under microscope. Results ： Compared with the positive control, the activities of ALT, AST and LDH were significantly reduced and the liver injury obviously attenuated in treated groups. Conclusion： The aqueous extract of Ficus hirta has an obvious protective effect against DMF-induced acute liver injury in mice.

关 键 词：五指毛桃 二甲基甲酰胺(DMF) 急性肝损伤 

分 类 号：R285.5[医药卫生—中药学]