五行汤抗肿瘤及经钙离子介导的促肿瘤细胞凋亡作用  

The Anti-tumour Effect of Wuxing Soup and its Mechanism in Inducing Apoptosis of Tumour Cells Mediated by Calcium

作　　者：莫非[1] 胡晶莹[2] 甘愉[2] 赵仰星[2] 赵新泰[2] 

机构地区：[1]复旦大学研究生院,上海200032 [2]上海市肿瘤研究所,上海200032

出　　处：《中药材》2008年第9期1368-1372,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:明确五行汤的抗肿瘤作用并初步探讨其机制。方法:不同浓度五行汤处理培养肿瘤细胞,MTT检测细胞存活率;流式细胞术检测细胞凋亡率;琼脂糖凝胶电泳检测细胞DNA变化;电镜观察细胞形态变化;特异性荧光探针检测胞浆钙离子浓度变化。结果:五行汤对多种肿瘤细胞生长有较特异的抑制作用;五行汤处理后的SGC-7901细胞经流式细胞仪检测出现亚二倍体峰,并呈时间-浓度依赖性;琼脂糖凝胶电泳呈现的DNA Ladder;电镜显示五行汤处理后的SGC-7901细胞呈现凋亡特征;F luo-3 AM染色荧光显微镜观察到胞浆钙离子浓度升高;胞内钙离子拮抗剂Bapta AM可以明显降低五行汤处理后SGC-7901细胞的凋亡率,且对细胞的保护作用呈浓度依赖性。结论:五行汤有较明确的抑制肿瘤细胞生长作用,可能通过钙离子介导的肿瘤细胞凋亡发挥作用。Objective ： To confirm the anti-cancer effect and mechanism of Wuxing soup. Methods ： Inhibition of cellular growth under Wuxing soup treatment was observed by MTT;Apoptosis was detected by gel electrophoresis, transmission electron microscopy and FACS ;The concentration of calcium was measured by fluorescence probe. Results： After SGC-7901cell being treated by Wuxing soup, it showed that： 1 ） Wuxing soup could specifically inhibit cancer cells proliferation in a time and dose dependent manner;2） Typical apoptotic morphological changes and DNA ladder of SGC-7901 cells were observed ;3 ） calcium inhibitor Bapta AM could reduce the apoptotic rate and protect SGC-7901 cells in a dose dependent manner. Conclusion： Wuxing soup has an effective inhibition on cancer cells, and can induce SGC-7901 cells to apoptosis by calcium.

关 键 词：五行汤 细胞凋亡 钙离子 

分 类 号：R285.5[医药卫生—中药学]