益气养肺方干预肺癌化疗耐药的线粒体及半胱天冬酶机制  

Effect of Supplement Energy and Nourish Lung Herbs on Drug-Resistance Lung Cancer with Mechanisms of Mitochondrial and Caspase Signal

作　　者：徐萌[1] 朱新海[1] 盛良翮[1] 

机构地区：[1]暨南大学附属第一医院肿瘤科,广东广州510630

出　　处：《中药材》2008年第9期1396-1399,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(30772844);广东省自然科学基金项目(5006066);中国临床肿瘤学科学基金项目(2005321)

摘　　要：目的:研究益气养肺方对肺癌耐药细胞体外生长和多药耐药相关蛋白(MRP)表达的影响,探讨益气养肺方干预肺癌耐药的线粒体及半胱天冬酶机制。方法:采用体外培养肺癌耐药细胞株SW 1573/2R120,分别以益气养肺方和阿霉素作用于肺癌细胞,通过MTT法检测细胞毒作用,流式细胞仪检测MRP蛋白表达,共聚焦显微镜测定线粒体膜电位和胞内钙离子(Ca2+)浓度,酶标法测定半胱天冬酶Caspase-3和Caspase-8活性。结果:与正常对照组相比,益气养肺方组细胞抑制率明显增加,MRP蛋白表达降低,线粒体膜电位和胞内Ca2+水平下降,Caspase-3和Caspase-8活性增加。结论:益气养肺方具有干预和逆转肺癌耐药的作用,其分子机制可能与下调线粒体膜电位水平和上调Caspase-3、Caspase-8活性有关。Objective ： To explore the effect of supplement energy and nourish lung（SENL） herbs on the growth of drug-resistance lung cancer cells, expression of muhidrug resistance-associated protein （MRP） and mechanisms of mitochondrial and Caspase signal. Methods ：Drug-resistance lung cancer cell line SW1573/2R120 was treated with SENL herbs and Adriamycin. Cellular toxicity with MTT colorimetric assay, expression of MRP protein with flow microscopy, the fluorescence intensity of mitochondria membrane potetional and Ca^2＋ with laser confocal microscopy, the activities of caspase-3 and caspase-8 with colorimetric assay were detected. Results ： Compared with control group, cellular inhibitory rate was increased obviously, expression of MRP protein was decreased, mitochondria membrane potetional and intracellular Ca^2＋ were decreased,the activities of caspase-3 and caspase-8 were increased in the group of SENL herbs. Conclusion ： SENL herbs can interfere and reverse drug resistance in lung cancer by the mechanism of decreasing mitochondria membrane potetional and increasing the activities of caspase-3 and caspase-8.

关 键 词：肺癌 多药耐药 半胱天冬酶 益气养肺方 

分 类 号：R285.5[医药卫生—中药学] R733.7[医药卫生—中医学]